梅花鹿骨提取物中2个新多肽的分离与鉴定

Two new polypeptides from extract of deer bone extract

利用C4300,Sephadex G-50和半制备HPLC等色谱技术对鹿瓜多肽注射液组方成分梅花鹿骨提取物的多肽类化学成分进行研究,分离纯化得到2个多肽类化合物,其精确相对分子质量分别为m/z 1 489.868 5和m/z 2 113.870 6。通过MALDI-TOF-MS及ExplorerTM软件从头测序（De Novo测序）分析,鉴定了2个多肽类化合物的N端（氮端）一级序列,分别为NH2-Gly-Pro-Val-Gly-Pro-Thr-Gly-Pro-Val-Gly-Ala-Ala-Gly-Pro-Se-Gly-Pro-Asp（梅花鹿骨18肽,1）和NH2-Ala-Gly-Pro-Ala-GlyPro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Pro-Asp-Ser-Try-Asp（梅花鹿骨23肽,2）。1和2均为新多肽。

Two new polypeptides were isolated and purified from the extract of deer bone（ constitutive part of Cucumis and Cervus polypeptide injection） by various column chromatography including C4300 and Sephadex G-50,as well as semipreparative HPLC.Their N-terminal amino acid sequences were identified by De Novo sequencing on the basis of MALDI-TOF-MS data and ExplorerTM software. The N-terminal amino acid sequences of polypeptides were identified as NH2-Gly-Pro-Val-Gly-Pro-Thr-Gly-Pro-Val-Gly-AlaAla-Gly-Pro-Ser-Gly-Pro-Asp（ Mei18 peptide,1） and NH2-Ala-Gly-Pro-Ala-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-GlyPro-Pro-Asp-Ser-Try-Asp（ Mei23 peptide,2）,respectively. Mei18 and Mei 23 peptides are new polypeptides.