HPLC-ABTS-DAD-Q-TOF/MS在线筛选黄连抗氧化活性物质

On-line scavenging activity of Huanglian by HPLC-ABTS-DAD-Q-TOF-MS

建立黄连特征化学成分和抗氧化活性相关联的二维指纹图谱,研究黄连抗氧化活性物质。利用高效液相多检测器联用的抗氧化活性成分在线检测体系,对黄连中化学成分进行检测,共鉴定出黄连中化学成分14种,其中具有抗氧化活性的成分8种;然后采用清除效率为指标对各活性成分的抗氧化活性进行评价。结果发现化合物2,3,4-三羟基苯丙酸、绿原酸、阿魏酸、枯拉灵、3-O-阿魏酰奎宁酸和阿魏酰基酪胺等具有较强的清除ABTS·＋的活性,而生物碱类成分对ABTS·＋的清除作用较弱,为黄连的抗氧化物质基础研究提供数据支持。

The present research aimed to establish an associated two-dimensional fingerprint of Huanglian between characteristic chemical composition and antioxidant activity,which was applied to on-line screen the active constituents. In this study,the HPLCABTS-DAD-Q-TOF/MS method,which can simultaneously identify individual components and rapidly screen for antioxidant compounds,was used to screen and identify antioxidant components in Huanglian. Fourteen compositions were discovered,and eight of them displayed antioxidant activity. The antioxidant activity of different ingredients was evaluated by antioxidant efficiency. The data showed that 2,3,4-trihydroxy phenylpropionic acid,chlorogenic acid,ferulic acid,cularine,3-O-feruloylquinic acid and feruloyltyramine showed stronger antioxidant activity than that of alkaloids. These experimental data can provide data support for the basic research of the antioxidant ingredients of Huanglian.