富角蛋白有机物对医院绿地真菌群落组成的影响

Influences of keratin-rich organic materials on fungal community composition in a hospital grassplot soil

将富含角蛋白的无菌鸡毛粉引入一医院绿地土后,基于高通量测序技术分析真菌群落的组成及相对丰度,发现在富含角蛋白基质鸡毛粉降解过程中共有真菌6门15纲39目71科128属。加入鸡毛粉不同时期(初期3d、中期30d、后期90d)的医院土真菌群落有较大变化。添加鸡毛粉3d的医院土(YY1)中真菌群落多样性丰富,优势种是芽殖久浩酵母Guehomyces pullulans(57.42%);加入鸡毛粉30d后(YY2),群落组成中不少种的相对丰度明显下降,优势种为一分类地位未定属,其相对丰度是77.57%;加入鸡毛粉90d后(YY3),一些潜在的人类病原菌的相对丰度有所上升,优势种是石膏样小孢子菌Microsporum gyseum(55.17%),而初期的优势种芽殖久浩酵母Guehomyces pullulans的相对丰度则明显降低。研究可知富含角蛋白的鸡毛粉对医院绿地土壤中的真菌群落,尤其是一些人体潜在病原真菌的群落组成和相对丰度有明显调节作用。

Based on high-throughout sequencing technology, the fungal community composition and the relative abundance of fungal species in a hospital grassplot soil were analyzed after adding sterile keratin-rich chicken feather powder. During degradation of sterile chicken feather powder in the soil, enriched fungal species were detected, belonging to 128 genera, 71 families, 39 orders, 15 classes and 6 phyla. The fungal communities greatly varied in three different periods（initial stage 3 d, mid-term 30 d and later stage 90 d） after addition of the sterile chicken feather powder. The diversity of fungal community in the samples was much richer in the initial stage（YY1） than that in other stages, and Guehomyces pullulans was found to be dominant（57.24%）. In the mid-term（YY2）, the relative abundances of species in the fungal community decreased, and anunclassified species was dominant, with relative abundance of 77.57%. The relative abundances of some potential human pathogens increased in the later stage（YY3）, the dominant species was Microsporum gypseum. The relative abundance of Guehomyces pullulans dominant in the initial stage obviously decreased in the later stage. The keratin-rich chicken feather powder had a significant regulatory effect on the composition and relative abundance of the fungi in hospital grassplot soil, especially on some human potential pathogenic fungi.