栀子—刺五加含药血清对大鼠海马神经元发生的影响

Effect of Gardenia Jasminoides-Acanthopanax Senticosus Containing Serum on Hippocampal Neurogenesis in Rats

目的观察栀子—刺五加含药血清对体外高浓度皮质酮作用下大鼠海马神经元发生的影响。方法 35只雄性Wistar大鼠按随机数字表分为栀子组(0.23g/d)、刺五加组(0.38g/d)、栀子—刺五加组(栀子0.23g/d,刺五加0.38g/d)、氟西汀组(0.51mg/d)、空白组,每组7只。连续灌胃10天后取含药血清和空白大鼠血清。体外建立高浓度皮质酮抑制大鼠海马神经元发生模型。采用MTT法测定神经元存活率,5-溴脱氧尿嘧啶核苷(BrdU)标记海马神经元增殖,神经元特异性烯醇化酶(NSE)标记海马神经元生长。结果与空白组及栀子组比较,栀子—刺五加组、刺五加组、氟西汀组海马神经元存活率明显提高(P<0.05),海马神经元中BrdU及NSE阳性细胞数明显增加(P<0.05)。结论栀子—刺五加含药血清和刺五加含药血清有利于海马神经元在高皮质激素环境下的存活、增殖、生长,具有促进海马神经元发生的作用。

Objective To study the effect of Gardenia jasminoides-Acanthopanax senticosus containing serum on hippocampal neurons in rats treated with high concentration of cortisol in vitro.Methods Totally 35 male Wistar rats were randomly divided into Gardenia jasminoides group（0.23 g/d）,Acanthopanax senticosus group（0.38 g/d）,Gardenia jasminoides-Acanthopanax senticosus group（Gardenia jasminoides 0.23 g/d,Acanthopanax senticosus0.38 g/d）,fluoxetine group（0.51 mg/d）,and the blank group,7 in each group.Ten days after continuous gavage,drug serum and blank rat serum were taken.The model of hippocampal neurons inhibited by high concentration of corticosterone was establishedin vitro.The survival rate of neurons was measured by MTT.The proliferation of hippocampal neurons was labeled by BrdU,and the growth of hippocampal neurons was labeled by neuron-specific enolase（NSE）.Results Compared with the blank group and the Gardenia jasminoides group,hippocampal neuron survival rate,and the positive cells number of BrdU and NSE increased in Gardenia jasminoides-Acanthopanax senticosus group,Acanthopanax senticosus group and fluoxetine group（P〈0.05）.Conclusion Gardenia jasminoides-Acanthopanax senticosus containing serum and Acanthopanax senticosus containing serum are beneficial to the survival,proliferation and growth of hippocampal neurons under the environment of corticosteroid with high concentration,and can promote the development of hippocampal neurons.