我国南海硇洲岛潮间带产油真菌的脂肪酸组成分析

Fatty acid composition analysis of oil producing fungi from intertidal zone of Naozhou island in South China Sea

采用尼罗红平板筛选法从我国南海硇洲岛潮间分离鉴定出的178株海洋真菌中筛选产油菌株,对其脂肪酸进行抽提并甲酯化,采用气相色谱-质谱联用(GC-MS)技术测定产油真菌的总脂肪酸含量并对其脂肪酸组成进行分析。结果表明:筛选出89株产油真菌,其中14株海洋真菌的总脂肪酸含量占冷冻干燥菌体质量的1.41%~7.73%,不饱和脂肪酸含量占总脂肪酸含量的42.51%~68.86%,多不饱和脂肪酸含量占总脂肪酸含量的9.03%~36.49%。我国南海硇洲岛潮间带产油真菌资源丰富,在生物油脂开发利用中具有较高价值,获取的不饱和脂肪酸具有广泛应用前景。

The oil producing strains were screened from the 178 strains marine fungi isolated from inter- tidal zone of Naozhou island in South China Sea using Nile red plates, then the fatty acid of the strains were extracted and methylated, and the total fatty acid content and fatty acid composition were analyzed by gas chromatography - mass spectrometry（ GC-MS）. The results showed that 89 strains of oil produ- cing fungi were screened. Among them, the total fatty acid content of 14 strains of oil producing fungi was 1.41% -7.73% of the mass of freeze- dried strains. The contents of unsaturated fatty acid and polyunsaturated fatty acid were 42.51% - 68.86 % and 9.03 % - 36.49 % respectively. The intertidal zone of Naozhou island in South China Sea was a rich resource for oil producing fungi, which had higher value in development and utilization of biolipid, and the unsaturated fatty acid obtained had wide appli- cation prospect.