育龄期女性缺铁性贫血患者血小板COX-1与COX-2的表达及临床意义

Expression of COX-1 and COX-2 in the Platelet of Iron Deficiency Anemia Women at Childbearing Age and Its Clinical Significance

目的:检测育龄期女性缺铁性贫血(iron deficiency anemia,IDA)患者血小板环氧化物酶1(cyclooxygenase-1,COX-1)和环氧化物酶2(cyclooxygenase-2,COX-2)的表达水平,并探讨其与贫血及血小板各指标间的相关性。方法:募集育龄期女性缺铁性贫血患者40例和健康女性对照组35例,采用流式细胞术分析血小板环氧化酶1和2(cyclooxygenase,COX-1,2)的表达,应用比浊法测定血小板聚集功能,电化学发光法检测血清铁蛋白水平,ELISA测定血清铁含量;采用SPSS19.0软件对各指标在2组中的差异及相关性进行统计分析。结果:与健康对照组相比,育龄期女性缺铁性贫血患者血小板COX-1和COX-2的表达水平显著降低(P<0.05),而血小板计数(Plt)、平均血小板体积(MPV)和血小板聚集率(PAgT)在2组间差异比较均无统计学意义(P>0.05)。相关性分析表明,血小板COX-1的表达水平与血红蛋白(Hb)(r=0.623,P<0.01)、血清铁(r=0.321,P<0.05)、红细胞压积(HCT)(r=0.305,P<0.05)均呈正相关,与Plt数、MPV和PAgT均无相关性(P>0.05);而血小板COX-2的表达水平与各指标间均不存在相关性(P>0.05)。结论:血小板COX-1,COX-2在育龄期女性缺铁性贫血患者中的表达水平明显下降,且COX-1与贫血严重程度密切相关,对育龄期女性缺铁性贫血的临床诊断具有参考价值。

Objective： To detect the expression level of cyclooxygenase-1（COX-1） and cyclooxygenase-2（COX-2） in the platelet of iron deficiency anemia（IDA）women at childbearing age and to explore its correlation with the different indexes of anemia and platelets. Methods： Forty female IDA patients at childbearing age and 35 healthy controls were enrolled in this study. The Flow cytometry was used to detect the expression of platelet COX-1 and COX-2,the platelet aggregation function as examined by turbidimetric method,and the levels of serum ferritin were analyzed by electrochemical luminescence method,the leval of serum iron was determined by ELISA,and the correlation of different indexes was analyzed. Results： Compared with healthy controls,the levels of platelet COX-1 and COX-2 were significantly lower in female IDA patients at Childbearing age（P〈0.05）,but platelet count（Plt）,mean platelet volume（MPV） and platelet aggregation rate（PAgT）were not statistically different between the 2 groups（P〈0.05）. The expression level of platelet COX-1 positively correlated with those of Hb（r = 0.623,P〈0.01）,serum iron（r = 0.321,P〈0.05） and HCT（r = 0.305,P〈0.05）. but the platelet COX-2 expression did not corelate with these indexs. Conclusion： The expression of platelet COX-1 and COX-2 in female IDA patients at Childbearing age markedly decrease,and the expression level of platelet COX-1 closely relates with the severity of anemia,that possesses reference value for clinical diagnosis of female IDA patients at Childbearing age.