环孢素A联合牙龈卟啉单胞菌脂多糖局部应用对大鼠牙周组织缺损修复影响研究

Effects of cyclosporine A and Porphyromonas gingivalis-lipopolysaccharide on defect repair in periodontal tissue in rats

目的探讨环孢素A(CsA)联合牙龈卟啉单胞菌脂多糖(P.g-LPS)局部应用对大鼠牙周组织缺损修复的影响。方法成功构建18只SD大鼠牙周急性缺损模型,随机均分为3组,分别在缺损对应口腔内局部注射生理盐水(对照组)、CsA 2 mg/kg(CsA组)或CsA 2 mg/kg+P.g-LPS 1μg(CsA+P.g-LPS组),30 d后处死全部大鼠,切取双侧下颌骨制作标本切片,HE染色观察CsA单独应用及与低剂量P.g-LPS联合应用对大鼠牙周组织缺损修复的影响。结果 CsA 2 mg/kg单独及与低剂量P.g-LPS联合应用,对大鼠牙周组织缺损的修复均有一定促进作用,但与对照组相比,差异均无统计学意义(P>0.05)。结论在模拟人体牙周炎恢复期低毒素、微炎症状态的牙周情况下局部应用CsA,既不会促进微炎症状态下的缺损修复,也不会与微炎症表现出协同作用而导致牙周组织的损害。

Objective To evaluate the effects of cyclosporine A（CsA）and Porphyromonas gingivalis-lipopolysaccharide（P.g-LPS）on defect repair in mandibular tissue in rats. Methods Saline,CsA（2 mg/kg）,CsA（2 mg/kg）＋P.g-LPS（1μg）were injected into the periodontal defects in Sprague Dawley rats. Thirty days after injection,animals were sacrificed,the formation of newly formed alveolar bone,cementum and the width of periodontal ligament were analyzed histomorphometrically. Results 2 mg/kg of CsA,used singly or in combination with low-dose P.g-LPS,had a certain effects on the regeneration of the periodontal tissue in Sprague Dawley rats,but the difference was not statistically significant compared with the control group. Conclusion The local application of CsA under the condition of low toxin and microinflammatory symptoms,during the recovery period of periodontitis,will not promote the repair of defects in the condition of microinflammation,nor will cooperate with microinflammation and cause damage to periodontal tissue.