摘要
The classic phytohormone auxin plays an essential role in priming meristematic cell differentiation in the shoot apical meristem to promote lateral organ initiation. Recently, several lines of evidence have suggested that auxin is not only transported to new primordia but also descends to the stem cells in the central zone. However, the function of auxin in stem cell regulation has remained elusive. Here, we show that auxin signaling in stem cells is mediated, at least in part, byAUXIN RESPONSE FACTOR 5/MONOPTEROS （ARF5/MP）, which directly represses the transcription of DORNROSCHEN /ENHANCER OF SHO0 T REGENERA TION 1 （DRN/ESR 1）. DRN expressed in stem cells positively regulates CLAVATA3 （CLV3） expression and has important meristematic functions. Our results provide a mechanistic framework for auxin control of shoot stem cell homeostasis and demonstrate how auxin differentially controls plant stem cell maintenance and differentiation.
The classic phytohormone auxin plays an essential role in priming meristematic cell differentiation in the shoot apical meristem to promote lateral organ initiation. Recently, several lines of evidence have suggested that auxin is not only transported to new primordia but also descends to the stem cells in the central zone. However, the function of auxin in stem cell regulation has remained elusive. Here, we show that auxin signaling in stem cells is mediated, at least in part, byAUXIN RESPONSE FACTOR 5/MONOPTEROS （ARF5/MP）, which directly represses the transcription of DORNROSCHEN /ENHANCER OF SHO0 T REGENERA TION 1 （DRN/ESR 1）. DRN expressed in stem cells positively regulates CLAVATA3 （CLV3） expression and has important meristematic functions. Our results provide a mechanistic framework for auxin control of shoot stem cell homeostasis and demonstrate how auxin differentially controls plant stem cell maintenance and differentiation.
作者
Linjie Luo
Jian Zengg
Haijun Wu
Zhaoxia Tian
Zhong Zhao
Linjie Luo;Jian Zeng;Haijun Wu;Zhaoxia Tian;Zhong Zhao(CAS Center for Excellence in Molecular Plant Sciences,School of Life Sciences,University of Science and Technology of China,Huangshan Road 443 Hefei 230027.China https：//doi.org/10.10164.molp.2018.04.006 Zhong Zhao（zhzhao@ustc.edu.cn)
基金
supported by the National Natural Science Foundation of China （31270325 and 91317310 to Z.Z. and 31400251 to H.W.） and the Ministry of Science and Technology of China （2013CB967300, to Z.Z.）. The authors thank Prof. Wolfgang Werr, Prof. Dolf Weijers, and Jan Lohmann for sharing mutant or transgenic seeds. RNA-seq data have been deposited in the Gene Expression Omnibus under accession number GEO： GSE93232. The authors declare no competing financial interests.
