白头翁汤正丁醇提取物对白念珠菌定植下溃疡性结肠炎小鼠的作用机制研究

Therapeutic potential of n-butanol extract of Pulsatilla decoction in a murine model of ulcerative colitis induced by DSS combined with Candida albicans colonization

研究白头翁汤正丁醇提取物（BAEB）对DSS诱导＋白念珠菌（CA）定植状态下的小鼠急性溃疡性结肠炎（UC）的作用机制。将小鼠随机分为正常对照组,DSS组,DSS＋CA组,BAEB高、中、低剂量组,阳性药Mesalazine组。观察小鼠一般情况,平板法检测小鼠肠道内容物真菌载荷量,HE染色观察小鼠结肠病理变化;ELISA法检测小鼠血清ASCA及结肠黏膜组织IL-6,IL-8,IL-1β,HBD-2,HBD-3的含量变化。结果表明,与DSS模型组相比,DSS＋CA组小鼠一般状况及血清ASCA无明显变化,但肠道CA载荷量、组织病理损伤程度、结肠黏膜组织IL-6,IL-8,IL-1β,HBD-2,HBD-3的蛋白水平均超过DSS组,BAEB高剂量与Mesalazine治疗能改善结肠组织病理,降低IL-6,IL-8,IL-1β,HBD-2,HBD-3的表达水平。上述结果提示,BAEB能有效改善DSS＋CA定植下的UC小鼠结肠病理及免疫性损伤,对肠道真菌过度定植相关肠炎的治疗具有重要价值。

To investigate the mechanism of n-butanol extract of Pulsatilla decoction（BAEB） against murine ulcerative colitis（UC）model induced by DSS combined with Candida albicans（CA） colonization,mice were randomly divided into normal control group,DSS group,DSS ＋ CA group,BAEB high,medium and low dose group,and positive drug Mesalazine group. The general condition of mice was observed,fungal loads of murine intestinal contents were detected by plate method,colonic pathological change of mice was examined by HE staining. ASCA in serum and IL-6,IL-8,IL-1β,HBD-2,HBD-3 in colonic mucosa were detected by ELISA. The results showed that,compared with DSS group,the general condition and ASCA in serum had no obvious change for DSS ＋ CA group,but the fungal loads in intestinal contents,the colonic pathological damage,and the levels of IL-6,IL-8,IL-1β,HBD-2,HBD-3 in colonic mucosa were greater than that of DSS group. High dose of BAEB group and Mesalazine group could improve the colonic pathology,decrease IL-6,IL-8,IL-1β,HBD-2,HBD-3 expression level. In conclusion,BAEB could effectively improve the UC symptoms in mice induced by DSS combined with CA colonization,and inhibit the inflammatory factors such as IL-6,imply that BAEB is of important value for the treatment of intestinal fungal-related colitis.