基于定量蛋白质组技术探索补阳还五汤治疗脑梗死气虚血瘀证的作用机制

Quantitative proteomic research of biological basis of Buyang Huanwu decoction therapy for cerebral infarction combined with Qi-deficiency and blood-stasis syndrome

中药＂补阳还五汤＂广泛应用于治疗脑梗死气虚血瘀证,但是其药理机制仍不明确。该研究应用非标记定量蛋白质组学技术探索＂补阳还五汤＂治疗脑梗死气虚血瘀证的生物学基础。以脑梗死气虚血瘀证大鼠模型为研究对象,比较假手术组、模型组、模型给药组大鼠脑组织的全蛋白表达水平。对3组样品进行定量分析,假手术组、模型组、模型给药组脑组织样品分别鉴定到3 959,3 996,4 055种蛋白。以模型组为对照,假手术组和给药组表达水平差异在2倍以上且趋势一致的蛋白共计391种。对差异表达蛋白进行生物信息学分析和功能聚类,发现补阳还五汤可能通过影响能量代谢、神经系统及一些信号通路蛋白的表达,从而对脑梗死气虚血瘀证发挥治疗作用。该研究从蛋白质水平探索了补阳还五汤发挥药效的生物学机制,为脑梗死气虚血瘀证的临床治疗和中医相关研究提供了分子依据。

Chinese medicine Buyang Huanwu decoction（ BYHW） is widely used in treating cerebral infarction combined with Qideficiency and blood-stasis syndrome,but the pharmacological basis is still not clear. This study aims to uncover the biological basis of BYHW therapy for cerebral infarction combined with Qi-deficiency and blood-stasis syndrome using label-free proteomic technology.Using Qi deficiency and blood stasis rat cerebral infarction model as the research object,the protein expression of rat brain tissue was compared among the sham operation group,the model group and the drug group. Quantitative analysis of the 3 groups of tissue samples detected 3 959,3 996 and 4 055 proteins in the sham operation group,the model group and the drug group,respectively. Take model group as the control group,391 proteins were identified to be upregulated or downregulated for more than 2 folds. Biological analysis and functional enrichment of the differentially expressed proteins revealed that BYHW may treat cerebral infarction combined with Qideficiency and blood-stasis syndrome through energy metabolism,nervous system and several signal pathways. This study preliminarily revealed the pharmacological mechanism of BYHW at the protein level,and provided a molecular basis for clinical treatment and traditional Chinese medicine research on cerebral infarction combined with Qi-deficiency and blood-stasis syndrome.