植物生长调节剂及氮素处理对拟巫山淫羊藿采后再萌发的影响

Effects of plant growth regulators and nitrogen on germination of Epimedium pseudowushanense after its stems and leaves reaped

为了探究适宜的促进拟巫山淫羊藿采后快速萌发、恢复生长的方案,选用6-BA、尿素、碳酸氢铵及GA3在采收后喷根处理,在不同的时期统计生物学指标,并采用高效液相的方法检测干燥成熟叶片中淫羊藿苷类黄酮成分的含量。结果表明6-BA 90 mg·L^（-1）（B1）,6-BA 60 mg·L^（-1）（B2）及综合1（6-BA 30 mg·L^（-1）＋尿素300 mg·L^（-1））、综合2（6-BA 60 mg·L^（-1）＋尿素300 mg·L^（-1））、综合4（6-BA 60 mg·L^（-1）＋碳酸氢铵300 mg·L^（-1））显著促进了采后芽萌发,使快速建立新的光合体系,在处理1个月时分枝数分别较对照组提高165.9%,115.76%,103.86%,104.50%,81.67%。但是6-BA 90 mg·L^（-1）（B1）,6-BA 60 mg·L^（-1）（B2）处理组第2年萌发的分枝数及总叶片产量较对照组显著降低;综合1、综合2、综合4处理虽然第2年萌发的分枝数与对照组相比无显著差异,但是所获得的平均每株叶片产量有显著增加,增幅为36.80%,32.84%,45.97%,且对其中的淫羊藿苷类有效成分没有显著影响,因此,该实验的综合1、综合2及综合4处理为较合适的促进拟巫山淫羊藿采收后再萌发的实验处理。

In order to explore appropriate measures to promote germination after the harvest of Epimedium pseudowushanense,6-BA,urea,ammonium bicarbonate and GA3 were chosen to spray on the root and rhizomes,and then the biological indicators such as branches,leaf length,leaf width,plant height and so on,were measured in different periods,and the contents of epimedin A,epimedin B,epimedin C and icariin in the dry leaves were detected by HPLC. Results showed that 6-BA 90 mg·L^（-1）（ B1）,6-BA 60 mg·L^（-1）（ B2）,6-BA 30 mg·L^（-1）＋ urea 300 mg·L^（-1）（ C1）,6-BA 60 mg·L^（-1）＋ urea 300 mg·L^（-1）（ C2）,6-BA 60 mg·L^（-1）＋ ammonium bicarbonate 300 mg·L^（-1）（ C4） significantly increased bud germination in the early period,and the plants quickly set up new system of photosynthesis,the branches in a month of which were higher than the control group respectively by 165. 9%,115. 76%,103. 86%,104. 50%,81. 67%. However the branches developed the next year and the dry weight of leaves per plant in group B1 and B2 were much lower than that in control group. The groups that use 6-BA and nitrogen at the same time reaped a good yield of leaves even though the treatment had no significant influence on the branches developed the next year. The dry weight of leaves of C1,C2,C4 treatments were 36. 80%,32. 84%,45. 97% more than the control group respectively. Therefore,C1,C2 and C4 treatments are the more appropriate to promote recovery after harvest. Furthermore,different groups,except 10 mg·L^（-1）6-BA treatment significantly reduced the content of epimedin C,other groups didn＇t have any significant effect on the contents of such flavonoids.