多花蒿中愈创木内酯类成分研究

Guaianolides from aerial parts of Artemisia myriantha

采用多种色谱技术对多花蒿干燥地上部分95%乙醇提取物进行化学成分研究,得到10个愈创木内酯类化合物,通过理化数据和波谱分析鉴定其结构分别为多花蒿内酯E（1）,tanaphillin（2）,1β,10β-epoxydehydroleucodin（3）,5-hydroxyleucodin（4）,dehydrocostuslactone（5）,3-O-methyl-iso-secotanapartholide（6）,roxbughianin A（7）,dehydroleucodin（8）,arglabin（9）,8α-acetoxyarglabin（10）。化合物1为新化合物,2~7为首次从该植物中分离得到。化合物3对人肝癌细胞Bel-7402较强的细胞毒活性,IC50为5.35μmol·L^（-1）,化合物6对人胃癌细胞（BGC-823）有较强的细胞毒活性,IC50为2.68μmol·L^（-1）。

This study was performed to investigate the guaianolides from the aerial parts of Artemisia myriantha. The chemical constituents were isolated by chromatographic columns over silica gel,Sephadex LH-20,and ODS,as well as Semi-prep HPLC methods,and their structures were identified by NMR and MS data. Ten compounds were isolated and identified as follows： artemyriantholide E（ 1）,tanaphillin（ 2）,1β,10β-epoxydehydroleucodin（ 3）,5-hydroxyleucodin（ 4）,dehydrocostuslactone（ 5）,3-O-methyl-iso-secotanapartholide（ 6）,roxbughianin A（ 7）,dehydroleucodin（ 8）,arglabin（ 9）,and 8α-acetoxyarglabin（ 10）. Compound 1 was a new compound,and compounds 2-7 were isolated from this plant for the first time. Compound 3 exhibited selective cytotoxicity against human liver cancer（ Bel-7402） with IC50 value of 5. 35 μmol·L^（-1）,and 6 against human gastric cancer（ BGC-823） with IC50 value of 2. 68μmol·L^（-1）,respectively.