益气活血方对糖尿病GK大鼠肾脏保护作用的研究

Protective effect of Yiqi Huoxue decoction on kidney in diabetic GK rats

研究益气活血方对糖尿病GK大鼠肾脏保护作用。通过8只雄性Wistar大鼠作为正常对照组,16只雄性GK大鼠按体质量、血糖值随机分为模型组、益气活血方组,每组8只,连续给药6周。每周称大鼠体质量,并测空腹血糖（FBG）;第3,6周末检测糖化血红蛋白值（Hb ALc）与24 h代谢值并收集尿液,生化法检测尿糖（U-GLU）、尿肌酐（U-CREA）、尿蛋白总量（U-TP）,计算24 h蛋白排泄率（UAER）;6周末,麻醉处死大鼠,心脏取血,生化法检测血清中肌酐（CREA）、尿酸（UA）及总胆固醇（CHOL）,手工法检测血清谷胱甘肽过氧化酶（GSH-Px）;取双肾,称重,计算肾肥大指数,将肾置中性甲醛,PAS染色,光镜下观察病理变化。结果显示,与正常组相比,模型组FBG,Hb ALc,24 h代谢值,U-GLU,UAER,血清CREA,UA,CHOL,肾肥大指数均显著性升高（P〈0.05）,体质量增长速度、血清GSH-Px均显著性降低（P〈0.05）,模型组大鼠肾病理切片有明显肾小球损伤及结构改变;与模型组相比,给药组大鼠血清GSH-Px显著性升高（P〈0.05）,FBG,Hb ALc,24 h代谢值,U-GLU,UAER,血清CREA,UA,CHOL,肾肥大指数显著性降低（P〈0.05）,给药组大鼠肾病理切片的肾小球损伤及结构改变有所改善。结果表明益气活血方可明显升高糖尿病GK大鼠的血清GSH-Px,降低FBG,Hb ALc,24 h代谢值,U-GLU,UAER,血清CREA,UA,CHOL,肾肥大指数,改善肾脏病理。

To investigate the protective effect of Yiqi Huoxue decoction on the kidney in diabetic goto-Kaizaki（ GK） rats. Eight Wistar rats were used as normal control group. According to body weight and blood glucose,16 GK rats were randomly divided into model control group and Yiqi Huoxue group,with 8 rats in each group. Drugs were administrated for 6 weeks. The rats were weighed and fasting blood glucose（ FBG） were measured weekly; the glycosylated hemoglobin（ Hb ALc） and 24 h metabolic rate were measured at the end of third and sixth weeks,at the same time,urine was collected to measure urinary glucose（ U-GLU）,urinary creatinine（ U-CREA） and total proteinuria（ U-TP） by biochemical method and calculate 24 h protein excretion rate（ UAER）. At the end of treatment,rats were sacrificed in anesthesia,and creatinin（ CREA）,uric acid（ UA） and cholesterol（ CHOL） were detected by biochemical method and glutathione peroxidase（ GSH-Px） was detected by manual method with the blood drawn from heart. Both kidneys were taken and weighed to calculate the renal hypertrophy index. The kidneys were placed into neutral formalin and observed under light microscope for pathological change after Periodic Acid-Schiff（ PAS） staining. The results showed that as compared with the normal control group,FBG,Hb ALc,24 h Metabolic rate,U-GLU,UAER,serum CREA,UA and CHOL levels,as well as renal hypertrophy index were significantly increased（ P 0. 05） in the model group,while the body weight growth rate,serum GSH-Px level were significantly reduced（ P 0. 05）; in addition,renal glomerular injury and structural changes were observed in the model group. As compared with the model group,serum GSH-Px level was significantly increased（ P 0. 05） in the Yiqi Huoxue group,While FBG,Hb ALc,24 h Metabolic rate,U-GLU,UAER,serum CREA,UA and CHOL levels,as well as renal hypertrophy index were significantly reduced（ P 0. 05）; meanwhile,renal glomerular injury and structural changes were improved in the Yiqi Huoxue group. The results showed that Yiqi Huoxue decoction can significantly increase serum GSH-Px level,reduce FBG,Hb ALc,24 h metabolic rate,U-GLU,UAER,serum CREA,UA and CHOL levels,as well as renal hypertrophy index,and improve renal pathology in diabetic GK rats.