化浊解毒方对慢性糜烂性胃炎浊毒内蕴证患者HIF-1α,VEGF,PTEN的影响

Effect of Huazhuo Jiedu formula on HIF-1α,VEGF,and PTEN in chronic erosive gastritis patients with syndrome of accumulation of turbidity and toxicity

观察化浊解毒方治疗慢性糜烂性胃炎浊毒内蕴证患者的临床疗效,并通过检测慢性糜烂性胃炎浊毒内蕴证患者治疗前后血清和胃黏膜组织中HIF-1α,VEGF,PTEN表达水平的变化,探讨化浊解毒方的作用机制,为浊毒理论在治疗慢性糜烂性胃炎的临床应用提供理论依据。70例慢性糜烂性胃炎患者随机分为对照组和治疗组各35例,对照组给予阿拉坦五味丸,每日2次,每次1袋,治疗组给予化浊解毒方口服,每日1剂,2组均治疗6个月。观察2组患者治疗前后临床症状、胃镜象及病理情况变化,以及2组治疗前后血清和胃黏膜组织HIF-1α,VEGF,PTEN表达水平的变化。结果治疗组临床疗效、胃镜疗效和病理疗效优于对照组（P〈0.05）;治疗后治疗组血清HIF-1α,VEGF水平均较对照组降低（P〈0.05）,血清PTEN水平较对照组升高（P〈0.05）;治疗后胃黏膜组织HIF-1α,VEGF表达水平均较对照组明显降低,胃黏膜组织PTEN表达水平较对照组升高,差异有统计学意义（P〈0.05）。结果表明,化浊解毒方能明显改善慢性糜烂性胃炎浊毒内蕴证患者的临床症状、胃镜象及病理,其作用机制是化浊解毒方可能通过降低慢性糜烂性胃炎胃黏膜组织HIF-1α,VEGF的表达水平,提高PTEN表达水平而起作用。

To observe the clinical efficacy of Huazhuo Jiedu formula in treating chronic erosive gastritis（ CEG） patients with syndrome of accumulation of turbidity and toxicity,explore its mechanism by observing the changes in expression levels of hypoxia inducible factor（ HIF-1α）,vascular endothelial growth factor（ VEGF） in serum and gastric mucosa tissues after treatment,and provide theoretical basis for the clinical application of Huazhuo Jiedu formula in treating chronic erosive gastritis. All 70 patient of CEG were randomly divided into control group and treatment group,35 cases in each group. The patients in control group received Alatan Wuwei Wan,bid,1 bag/time; while the patients in treatment group were given with Huazhuo Jiedu formula,1 dose/day. The course of the treatment was 6 months in both groups. The changes in clinical symptoms,gastroscopic signs,pathology and the expression levels of HIF-1α,VEGF,and phosphatase and tensin homolog deleted on chromosome ten（ PTEN） in serum and gastric mucosa tissues were observed in both groups. The results showed that treatment group was better than control group in clinical efficacy,gastroscopic efficacy and pathological effect after treatment（ P 0. 05）; the levels of HIF-1α and VEGF in serum of treatment group were lower than those in the control group after treatment（ P 0. 05）,while the level of PTEN in serum of treatment group was higher than that in the control group after treatment（ P 0. 05）; the levels of HIF-1α and VEGF in gastric mucosa tissues in the treatment group were lower than those in the control group after treatment,while the level of PTEN in gastric mucosa tissues in treatment group was higher than that in the control group after treatment（ P 0. 05）,with statistically significant differences between these two groups（ P 0. 05）. Huazhuo Jiedu formula can improve the clinical symptoms,gastroscopic signs and pathological conditions in CEG patients with syndrome of accumulation of turbidity and toxicity,and the mechanism may be associated with decreasing the expression level of HIF-1α,VEGF and increasing the expression level of PTEN.