半抗原修饰的肿瘤疫苗治疗小鼠T淋巴细胞瘤效果观察

Treatment of T lymphoma with hapten modified tumor vaccine

目的 :探讨用半抗原修饰的瘤苗治疗小鼠T淋巴细胞瘤的方法。方法 :将C57BL/ 6小鼠随机分为灭活RMA(小鼠T淋巴细胞瘤来源的细胞系 )肿瘤细胞组 ,二硝基苯 (DNP)修饰的灭活RMA肿瘤细胞组 ,RMA肿瘤细胞破碎物组 ,DNP修饰的RMA肿瘤细胞破碎物组和生理盐水对照组共 5组 (每组 1 0只 ) ,于免疫接种后第 1 0d用RMA肿瘤细胞再攻击实验小鼠 ,观察它们抵抗RMA肿瘤细胞再攻击的能力 (主要观察出瘤时间 ,肿瘤生长速度和生存期 )。结果 :DNP修饰的灭活PMA肿瘤细胞可诱导小鼠产生抗肿瘤免疫保护力 ,主要表现为小鼠肿瘤生长速度减慢 ,生存期延长 ;而灭活RMA肿瘤细胞、RMA肿瘤细胞破碎物和DNP修饰的肿瘤组织破碎物不能诱导小鼠产生抗肿瘤免疫保护力。结论

Aim: To study the method for treating mouse T lymphoma with hapten modified tumor vaccine. Methods: The C57BL/6 mice were randomly allocated into 5 groups(10 mice in each group): inactivated RMA (cellinage of mouse T cell lymphoma) tumor cell, DNP modified inactivated RMA tumor cell,broken RMA tumor cell, DNP modified broken RMA tumor cell, and normal saline groups.On the 10th day after vaccination, they were re attacked with RMA tumor cell to induce the anti tumor immune reaction.The tumor occur time, tumor volume, and survival time were observed. Results: DNP modified inactivated RMA tumor cells could induce anti tumor immune reaction and the mice had a lower tumor growth speed and a longer survival time after re attacked with RMA;whereas the inactivated RMA tumor cells,RMA broken tumor cells and the DNP modified tumor cell broken matters could not. Conclusion: Hapten modified tumor vaccine may be an effective anti tumor vaccine.