高唐栝楼茎尖分生组织培养与快速繁殖技术研究

Technology System for Stem-apex Meristem Culture and Rapid Propagation of Trichosanthes kirilowii Maxim.

[目的]采用茎尖分生组织培养脱毒技术培育高唐栝楼的脱病毒试管苗。[方法]对添加不同浓度外源生长调节剂的培养基进行一系列优化试验,筛选出高唐栝楼脱毒试管苗最佳繁殖培养基。[结果]在MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0.10 mg/L KT培养基中诱导效果较好,技术上达到了快速繁殖规模生产的要求。经反复的病毒检测,证明脱毒试管苗脱病毒彻底。[结论]最终获得了高唐栝楼茎尖培养脱毒与诱导、增殖和生根的培养基,及其生长所需要的外部环境条件和相关配套技术。

[ Objective ] Virus-free seedling in test tube of Trichosanthes kirilowii Maim. was obtained from stem-apex meristem culture invitro. [ Method ] A series of optimization experiments sor propagative medium composition and with a view to accclerate the propagation of virus-free seedling in test tube of T. kirilowii Maxim. [Result]The most suitable rapid propagative medium of T. kirilowii Maxim, was MS＋2. 0 mg/L 6-BA ＋0. 1 mg/L NAA＋0. 10 mg/L KT. These results could meet the technology require-ments of rapid propagation in large scale production. Virus-free seedlings in test tube were tested repeatedly ,which had no virus completely.[Conclusion ] The cultural mediums of detoxification and induction,proliferation and rooting were obtained as well conditions for the plant growth and matching technology.