泌尿生殖道支原体感染检测、鉴定及耐药性分析

Detection,Identification and Drug Resistance Analysis of Urogenital Mycoplasma Infection

目的探究泌尿生殖道支原体感染情况及对常用抗菌药物的耐药情况,为指导临床合理应用抗菌药物提供科学依据。方法本研究使用支原体培养、鉴定及药敏检测试剂盒(液基型),对临床收集的489例疑似泌尿生殖道感染患者的标本进行支原体培养、鉴定及药敏检测。结果发现在489例标本中,支原体阳性有202例,阳性率为41.30%,人型支原体单一阳性有24例,占11.88%,解脲支原体单一阳性有171例,占84.65%,混合感染有7例,占3.47%。女性、男性支原体检出率分别为52.36%、17.44%,女性明显高于男性。阳性患者以20~35岁为主,占66.83%。支原体对交沙霉素、强力霉素和美满霉素敏感性最高,敏感率分别为89.60%、88.12%和84.65%,对红霉素、司巴沙星、环丙沙星、氧氟沙星和左氧氟沙星敏感性较低,敏感率分别为18.81%、10.40%、1.49%、6.44%和5.94%。结论本研究证实支原体感染中主要由解脲支原体感染为主。临床应参考药敏结果合理用药,经验用药时交沙霉素、强力霉素和美满霉素最好,应避免使用司巴沙星、环丙沙星、氧氟沙星和左氧氟沙星。

Objective To investigate the infection of urogenital mycoplasma and the resistance to common antibiotics,and to provide scientific basis for guiding the rational application of antibiotics.Methods Mycoplasma culture,identification and drug sensitivity test kits（liquid-based）were used to culture,identify and detect drug susceptibility in 489 patients with suspected genitourinary tract infections.Results The results showed that among 489 specimens,202 were positive for mycoplasma, the positive rate was 41.30%,24 were positive for human mycoplasma,accounting for 11.88%,there were 171 cases of ureaplasma urealyticum single,accounting for84.65%,and 7 cases of mixed infection,accounting for 3.47%.The detection rates of female and male mycoplasma were 52.36% and17.44%,respectively,and women were significantly higher than men.Positive patients were mainly 20 to 35 years old,accounting for66.83%.Mycoplasma is most sensitive to josamycin,doxycycline and minocycline,with sensitivity rates of 89.60%,88.12% and 84.65%,respectively,sensitivity to erythromycin,sirfloxacin,ciprofloxacin,ofloxacin and levofloxacin was low,with sensitivity rates of 18.81%,10.40%,1.49%,6.44% and 5.94%,respectively.Conclusion This study confirmed that mycoplasma infection is mainly caused by ureaplasma urealyticum infection.Clinical reference should be made to the rational use of drug susceptibility results.It is best to use josamycin,doxycycline and minocycline when using drugs.Sparfloxacin,ciprofloxacin,ofloxacin and levofloxacin should be avoided.