响应面优化超声提取香芹茎叶维生素C工艺研究

Optimization of Ultrasound Extraction of Vitamin C in the Stems and Leaves of Libanotis seseloides by Response Surface Methodology

本文以香芹为研究对象,2%草酸溶液为提取剂,采用单因素试验和响应面分析法优化提取香芹茎、叶中维生素C的工艺条件,考察液料比、超声温度和超声时间对其维生素C提取量的影响,运用高效液相色谱(HPLC)法测定香芹茎、叶中维生素C的含量。结果表明,香芹茎叶中维生素C最佳工艺分别为:液料比6mL/g、超声温度40℃、超声时间22min和液料比8mL/g、超声温度43℃、超声时间21min,测得香芹茎、叶中维生素C的含量分别为0.065mg/g,0.842mg/g,可见:香芹叶中的维生素C含量明显高于其茎中的,在叶中的含量约为茎中的13倍。

Libanotis seseloides as the study object in this paper, 2% oxalic acid as extractant, Effects of material to solvent ratio, ultrasonic temperature and ultrasonic time on the extraction of Vitamin C were studied by single factor and response surface methodology, analyzed with high performance liquid chromatography（HPLC）. The optimal extraction technology was as follow： material to solvent ratio 6 mL/g, ultrasonic temperature 40℃, ultrasonic time 22 min, and material to solvent ratio 8 mL/g, ultrasonic temperature 43℃, ultrasonic time 21 min in Libanotis seseloides stems and leaves, respectively. The vitamin C content 0.065 mg/g, 0.842 mg/g in Libanotis seseloides stems and leaves, respectively. Its content in the leaves is about 13 times in the stems.