不同无机盐浓度对金霉素发酵生产影响

目的:通过对金霉素发酵配方中各无机盐配比进行优化,筛选最有利于金霉素生产的浓度比例提高金霉素发酵效能。方法:通过利用部分因子设计方法设计出不同组合的无机盐配方进行初筛试验筛选对金霉素发酵效能有促进作用的几个无机盐配比组合,综合考虑无机盐组分和其它成分的影响,采用响应曲面优化方法进一步优化金霉素发酵无机盐配方。结果:优化后金霉素发酵基础配方中无机盐配比为氯化钠0.23%,硫酸铵0.63%,碳酸钙0.57%,硫酸镁0.025%,磷酸二氢钾0%,且单批金霉素发酵效价及正放产量较初始配方条件分别提高852μ/ml和261kg,实现增产增效的目的。同时发现不同硫酸铵浓度对菌丝形态有一定影响。结论:不同无机盐浓度对金霉素发酵有一定影响,经优化后金霉素发酵效能得到进一步提高。

Objective：to optimize the proportion of inorganic salts in chlortetracycline fermentation formula,and to select thebest concentration ratio for chlortetracycline production to improve the efficiency of chlortetracycline fermentation. Methods：differentcombinations of inorganic salts were designed by using partial factor design method to screen out several combinations of inorganicsalts which could promote the fermentation efficiency of chlortetracycline. Considering the effects of inorganic components and othercomponents,response surface methodology was used to further optimize the formula of chlortetracycline fermentation inorganic salts.Results：the optimized basic formula of chlortetracycline fermentation was composed of sodium chloride 0.23%,ammonium sulfate0.63%,calcium carbonate 0.57%,magnesium sulfate 0.025%,potassium dihydrogen phosphate 0%. 852 滋∕ml and 261 kg. At thesame time,it was found that different concentration of ammonium sulfate had some effect on the morphology of mycelium. Conclu-sion：different concentrations of inorganic salts have some effects on the fermentation of chlortetracycline,and the fermentation effi-ciency of chlortetracycline can be further improved after optimization.