4种红肉苹果提取液花青苷含量及体外抗氧化研究

Anthocyanin Content and Antioxidant Activity in Vitro of Red Flesh Apple Extracts

以红肉苹果‘新疆1号’,‘红勋1号’,‘黛红’和‘红爱’在幼果期,发育期和成熟期果实的HCL/甲醇提取液为试材,测定其花青苷含量及体外抗氧化性能。结果:‘新疆1号’幼果期果肉提取液颜色鲜红,花青苷含量最高,为680mg/kg,‘红爱’果肉提取液中花青苷含量是‘黛红’的10倍。不同品种/品系红肉苹果发育时期果肉提取液对DPPH自由基的清除率均较高,显著高于VC的清除能力,对羟基自由基和超氧阴离子的清除率则较低。其中‘红爱’和‘新疆1号’对DPPH的清除率随提取液浓度的升高而增加,分别由25%和49%增加到76%和85%;‘黛红’和‘红勋1号’对DPPH自由基的清除率随提取液浓度的变化保持在80%~90%。发育期‘黛红’果肉提取液浓度为10mg/kg时,对三种氧化自由基的清除上都高于‘红爱’。不同红肉苹果品种/品系的不同发育时期提取液中花青苷含量差异极大,对DPPH自由基的清除能力最强。

The HCL/methanol extracts from fruits of red flesh apple‘Xinjiang No.1＇,‘Hongxun No.1＇,‘Daihong＇and‘Red love＇at different developmental stages including young,developmental and mature period were used as test materials to determine anthocyanin content and antioxidant activity in vitro.Result：The young fruit extract of‘Xinjiang No.1＇with bright red color had the highest anthocyanin contentthat is 680 mg/kg.In addition,the anthocyanin content of‘Red love＇was ten times of＇Daihong＇.The DPPH free radical scavenging rate of pulp extracts was all significantly higher than that of VC scavenging ability in different cultivars/strains of red flesh apple during the developmental period.However,the clearance rate of hydroxyl radicals and superoxide anions were lower than that of DPPH.The removal rates of DPPH from ‘Red Love＇and ‘Xinjiang No.1＇increased with the increasing of extracts concentration,which increased from 25% and 49%to 76% and 85%,respectively;and the clearance rate of‘Daihong＇and‘Hongxun No.1＇on the DPPH radical scavenging rate was maintained at 80%to 90%.When content of anthocyanin was 10 mg/kg,the scavenging rate of three free radicals of developmental‘Daihong＇was higher than that of‘Red love＇.The content of anthocyanin in the extracts of different red flesh apple varieties/strains at different developmental stages varied greatly,and the scavenging ability of DPPH free radicals was the strongest.