咬合创伤大鼠模型中肌损伤蛋白含量的变化

Changes of Protein Content of Muscle Injury in Rat Model of Occlusion Trauma

目的:观察大鼠咬合创伤后咬肌肌酸激酶和乳酸脱氢酶含量的变化,探讨咬合创伤对咬肌组织的影响。方法:选用25只8周龄健康雄性SD大鼠,随机分为对照组(5只)和实验组(20只),实验组再分为4个亚组,即3d、7d、10d、14d组,每组5只大鼠。实验组用粘接剂将正畸不锈钢方丝粘固于左侧上颌第一磨牙牙合面,对照组不做任何处理。咬合创伤后3d、7d、10d、14d处死大鼠,通过全自动生化仪检测血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)的含量。结果:与对照组相比,咬合创伤7d后大鼠血清中CK、LDH含量显著升高(P<0.05),咬合创伤3d、10d、14d血清中CK、LDH含量没有显著改变(P>0.05)。结论:咬合创伤后大鼠血清中CK、LDH含量增高,但随着时间的延长,CK、LDH含量可逐渐恢复正常。

Objective：The changes of creatine kinase（CK）and lactate dehydrogenase（LDH）in the masseter muscle after occlusal trauma were observed to investigate the effect of occlusal trauma on masseter muscle tissue.Methods：25 healthy 8-week-old male SD rats were randomly divided into control group（n=5）and experimental group（n=20）.The experimental group was further divided into four subgroups,namely 3 d,7 d,10 dand 14 dgroups,with five rats in each group.In experimental group,metal-wire was attached to the left maxillary first molar by rein while the control group did not do any treatment.The rats were sacrificed at 3 d,7 d,10 d,14 dafter occlusion and the contents of CK and LDH in serum were detected by automatic biochemical analyzer.Results：Compared with the control group,the levels of CK and LDH in the serum of the rats were significantly increased after 7 days of occlusal trauma（P〈0.05）,and the contents of CK and LDH in the serum of the occlusal trauma were not significantly changed at 3 d,10 d,and 14 d（P〉0.05）.Conclusion：The contents of CK and LDH in the serum of rats increased after occlusal trauma,but with the prolongation of time,the contents of CK and LDH gradually returned to normal.