枫香根中水晶兰苷定性定量分析方法研究

Study on Qualitative and Quantitative Analysis of Monotropein in Liquidambaris Radix

目的建立枫香根中水晶兰苷的定性定量分析方法。方法采用薄层色谱法对枫香根中水晶兰苷进行定性鉴别。采用HPLC测定样品中水晶兰苷含量,色谱柱为依利特Sino Chrom ODS-BP柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸溶液(1∶99),检测波长为235 nm,流速为1.0 m L/min,柱温为25℃,进样量为10μL。结果薄层色谱法可鉴别出水晶兰苷。水晶兰苷在0.150 1~1.501 0μg(r=0.999 8)范围内线性关系良好,平均回收率为95.93%(RSD=0.60%)。10个不同产地枫香根中水晶兰苷含量在0.20%~1.15%之间。结论该方法操作简便、稳定可靠、重复性好,可作为枫香根药材质量控制方法。

Objective To establish qualitative and quantitative methods for analyzing monotropein in Liquidambaris Radix. Methods Monotropein in Liquidambaris Radix was identified by using TLC. The contents of monotropein in the samples were determined by HPLC on an Sino Chrom ODS-BP column（4.6 mm × 250 mm, 5 μm） with methanol-0.1% phosphoric acid solution（1：99） as the mobile phase at the flow rate of 1.0 m L/min; the detection wavelength was 235 nm; the column temperature was 25 ℃; the injection volume was 10 μL. Results Monotropein in Liquidambaris Radix could be identified by TLC. Monotropein showed good linear relation in the range of 0.150 1– 1.501 0 μg（r=0.999 8）, and the average recovery rate was 95.93%（RSD=0.60%）. The contents of monotropein in Liquidambaris Radix from 10 different producing areas were among the range of 0.20%–1.15%. Conclusion The method is simple, stable, reliable and reproducible, which can be used for the quality control of Liquidambaris Radix.