大鼠睾丸内一氧化氮合酶的表达

The Expression of Nitric Oxide Synthase in Testes of Male Rat

目的 :阐明一氧化氮合酶 (NOS)在睾丸内的表达 ,探讨一氧化氮 (NO)在睾丸生殖功能中的作用。 方法 :取雄性SD大鼠睾丸于 4 %多聚甲醛中固定 ,常规石蜡切片。用免疫组化ABC法检测成年大鼠睾丸NOS的表达和定位。 结果 :内皮型NOS(eNOS)、神经型NOS(nNOS)、诱导型NOS(iNOS)在睾丸间质细胞内均有表达 ,精曲小管周围肌样细胞、血管内皮细胞和平滑肌细胞内仅为eNOS免疫反应阳性 ,而血管外膜纤维内仅有nNOS表达。免疫反应物质主要定位于细胞质 ,胞核为阴性。在生精细胞内 3种NOS免疫反应均为阴性。 结论 :3种NOS在睾丸内均有表达 。

Objectives: To determine the expression of nitric oxide synthase (NOS) in testis and to investigate the effects of NO on the reproductive function of testis. Methods: Testes of adult male Sprague Dawley rats were fixed in 4% paraformaldehyde. The paraffin sections were made as routine. Immunohistochemical ABC method was used to observe the localization of NOS. Results: Endothelia NOS (eNOS), neuronal NOS (nNOS) and inductive NOS (iNOS) were all expressed in Leydig cells.Only eNOS was expressed in peritubular myoid cells, endothelial and smooth muscle cells of blood vessel,while only nNOS expressed in tunica adventitia of testicular blood vessels.The reactive substance distributes in cytoplasm with negative nuclei. Immunoreactivity for eNOS,nNOS and iNOS in all spermatogenic cells was negative. Conclusions: Three kinds of NOS were all expressed in testis and the distribution of different NOS had a little difference.