新型石墨烯量子点在视网膜母细胞瘤成像中的作用研究

Experimental studies on the application of graphene quantum dots in retinoblastoma imaging

目的探讨叶酸表面功能化修饰N掺杂石墨烯量子点(FN-GQDs)在视网膜母细胞瘤细胞标记及成像中的作用和生物安全性评估。方法荧光显微镜检测FN-GQDs在视网膜母细胞瘤细胞株(Y79)体外培养中的标记、成像能力。构建裸鼠皮下瘤模型,检测FN-GQDs在体内对皮下Y79瘤体组织的靶向成像能力。并分别采用CCK-8和组织切片染色评估FN-GQDs在细胞体外培养和动物体内的生物安全性。结果荧光显微镜检测结果显示FN-GQDs可成功标记体外培养的Y79细胞株,并实现持续、稳定的荧光成像。裸鼠皮下Y79瘤模型的实验结果显示:FN-GQDs可通过血液循环向瘤体组织聚集,实现肿瘤的在体靶向成像。CCK-8和组织切片染色显示,FNGQDs组相较于生理盐水对照组,无明显的细胞毒性和体内系统毒性(P>0.05)。结论 FN-GQDs实现了对Y79体外细胞特异性标记与体内瘤体组织的靶向性成像,为视网膜母细胞瘤的早期诊断与筛查提供了非常具有前景的新方法。

Objective To investigate the effect and biosafety of FN-GQDs modified by folic acid on the retinoblastoma（ Y79）. Methods Confocal was used to examine the fluorescence properties of FN-GQDs and their labeling and imaging capabilities in Y79 cells. The nude mice were constructed to detect the targeted imaging ability of FN-GQDs in vivo on Y79 tumor tissues. Biosecurity of FN-GQDs at both cellular and animal levels was assessed by CCK-8 and Tissue Slice Staining. Results Confocal results showed that FN-GQDs successfully labelled the Y79 cell line for stable fluorescence imaging. The nude mice model of hypodermic tumor showed that FN-GQDs can accumulate to the tumor tissue through the blood circulation and realize the in-vivo imaging of the tumor. CCK-8 and histological sections showed that FN-GQDs had no significant cytotoxicity（ P〉0. 05） and no toxicity in vivo compared with the control group. Conclusions FN-GQDs can achieve specific labeling and imaging of retinoblastoma（ Y79） in vitro and in vivo,it provides a theoretical basis for the early diagnosis and screening for intraocular malignant tumor.