柠条组织培养体系的建立

Establishment of Tissue Culture System of Caragana intermedia

为了建立柠条的组织培养体系,用KF/MS加激素培养法,选择不同的消毒剂,对柠条茎段愈伤组织诱导,并进行芽分化、芽的继代增殖和生根培养。结果表明,次氯酸钠的消毒效果最好,最适浓度为2%,且浸泡外植体的最佳时间为8 min;诱导芽分化的基本培养基以KF+6-BA 0.8 mg/L+NAA 0.3 mg/L+蔗糖20 g/L+琼脂7 g/L为宜,继代增殖最适培养基配方为KF+6-BA 1.2 mg/L+NAA 0.3 mg/L+蔗糖20 g/L+琼脂7 g/L,适合生根的最佳培养基为1/2 MS+NAA 0.3 mg/L+蔗糖30 g/L+琼脂7 g/L+活性炭0.5 g/L。

To establish tissue culture system of Caragana intermedia, the stem section callus was induced by Murashige and Skoog（MS）and KF Streptococcus Agar（KF）culture medium supplemented with hormone and sterilized with the different disinfectants. The bud callus was carried on bud differentiation, bud subculture proliferation and root culture. The results indicated that the best method of disinfection for explant of Caragana intermedia tissue culture was treated with 2% Sodium Hypochlorite for 8 min. The basic culture medium of callus induction was KF ＋6-BA 0.8 mg/L ＋NAA 0.3 mg/L ＋sucrose 20 g/L ＋agar 7 g/L. The effect of proliferation subculture in KF＋6-BA 1.2 mg/L＋NAA 0.3 mg/L＋sucrose 30 g/L＋agar 7 g/L of culture medium was better. The optimum rooting medium was 1/2 MS＋NAA 0.3 mg/L＋sucrose 30 g/L＋agar 7 g/L＋acticarbon 0.5 g/L.