孕期哺乳期染氟对仔鼠学习记忆及氨基酸类神经递质的影响

Effects of fluorine on learning memory and amino acid neurotransmitters in pregnant and lactating rats

目的探讨染氟在孕期哺乳期对仔鼠学习记忆能力及氨基酸类神经递质谷氨酸(Glu)、γ氨基丁酸(GABA)的影响。方法选取雌性大鼠24只,对妊娠期和哺乳期(仔鼠生后3周)大鼠染氟,将孕鼠随机分组:对照组(正常饮食喂养),低氟组(5 mg/kg),高氟组(20 mg/kg),每组8只孕鼠。继续染氟喂养仔鼠至6周神经系统发育完全,每组保留25只仔鼠。仔鼠6周时进行Morris水迷宫定位航行实验和跳台实验,用高效液相色谱法检测血清、海马组织中谷氨酸Glu、г氨基丁酸GABA含量,采用硫代巴比妥酸法、黄嘌呤氧化酶法分别检测海马组织中丙二醛MDA、超氧化物歧化酶SOD含量。结果高氟组定位航行实验潜伏期比对照组明显延长,差异有统计学意义(P<0.05)。高氟组跳台实验潜伏期比对照组明显延长,差异有统计学意义(P<0.05)。三组血清中Glu、GABA分别比较,差异无统计学意义。高氟组Glu、GABA、SOD比对照组明显降低,MDA明显增高,差异有统计学意义(P<0.05)。结论孕期哺乳期染氟,导致子代学习记忆减退,氨基酸类神经递质含量下降。

Objective To investigate the effects of fluorine on the learning and memory and the amino acid neurotransmitter glutamate（ Glu） and GABA in pregnant and lactating rats. Methods Twenty four female rats during pregnancy and lactation（ 3 weeks after delivery） were randomly grouped as three groups： the control group（ normal diet）,low fluorine group（ 5 mg/kg）,high fluoride group（ 20 mg/kg） with 8 rats each group. All pregnant mice began to dye fluorine from GD0 to 100 mg/L solution prepared by sodium fluoride until the offspring were born,breastfeeding the offspring for 3 weeks. The development of the nervous system was complete at 6 weeks,with 25 mice left in each group.Morris water maze navigation experiment and platform experiment were carried out at 6 weeks among offspring rats using high performance liquid chromatography（ HPLC） method to detect serum and hippocampus glutamic acid Glu,GABA,and using glucosinolates barbituric acid method,xanthine oxidase method to detect malondialdehyde MDA in the hippocampus,superoxide dismutase（ SOD） content respectively. Results The incubation period of high fluorine group was significantly longer than that of the control group（ P 〈0. 05）,and the difference was statistically significant. The incubation period of the high fluorine group was significantly longer than that in the control group,and the difference was statistically significant. There was no statistically significant difference between Glu and GABA in three groups of serum（ P 〈0. 05）. Glu,GABA and SOD of high-fluorine group were significantly lower than those in the control group,and the MDA significantly increased,（ P〈 0. 05）,and the difference was statistically significant. Conclusion The study memory and the content of amino acid neurotransmitter decreased in pregnant and lactation period.