芪参二莲汤对肝纤维化大鼠基质金属蛋白酶-1及抑制因子的影响

Effects of Qishenerlian Decoction on Matrix Metalloproteinase-1 and Its Inhibitory Factors in Rats with Hepatic Fibrosis

目的研究不同剂量芪参二莲汤对肝纤维化大鼠基质金属蛋白酶-1及抑制因子变化的影响。方法于2017年6月~8月选择90只SD雄性大鼠作为研究对象,按照体质量随机分为6组,每组15只,其中1组为正常组,其余5组均为肝纤维化模型,将其分为模型组、秋水仙碱组以及芪参二莲汤低剂量组(5 g/kg)、中剂量组(10 g/kg)和高剂量组(15 g/kg);各给药组分别给予相应的药物灌胃,正常组、模型组则给予生理盐水灌胃;对比各组大鼠MMP-1和TIMP-1水平以及肝纤维化指标水平。结果正常组与模型组比较,后者的MMP-1、MMP-1/TIMP-1水平明显降低,而TLISA-1水平则明显升高,差异有统计学意义(P<0.05);模型组与各给药组比较,各给药组的MMP-1、MMP-1/TIMP-1水平较模型组升高,而TIMP-1水平则较模型组降低,差异有统计学意义(P<0.05),且给药组中,以芪参二莲汤高剂量组的变化水平最为显著,差异有统计学意义(P<0.05)。结论15 g/kg高剂量的芪参二莲汤用于治疗肝纤维化大鼠,能够有效促进MMP-1表达、减少TIMP-1表达,进而起到逆转肝纤维化的作用。

Objective To study the effects of different doses of Qishenerlian Decoction on matrix metalloproteinase-1 and inhibitor of hepatic fibrosis in rats.Methods In June to August 2017, 90 male SD rats were selected as the research subjects.According to the body mass,they were randomly divided into 6 groups,with 15 rats in each group,of which 1 was the normal group and the other 5 groups were liver fibrosis models.They were divided into model group,colchicine group and low dose group of Qishenerlian Decoction（5 g/kg）,medium dose group（10 g/kg）and high dose group（15 g/kg）.Each drug-administered group was given the corresponding drugs,and the normal group and the model group were given normal saline.The levels of MMP-1 and TIMP-1 and liver fibrosis were compared between the groups.Results Compared with the normal group,the level of MMP-1 and MMP-1/TIMP-1 in the model group was significantly decreased,while the level of TLISA-1 was significantly higher,the difference was statistically significant（ P〈0.05）;Compared with the drug-administered group,the MMP-1 and MMP-1/TIMP-1 levels in the model group were higher than those in the model group,while the TIMP-1 level was lower than that in the model group,the difference was statistically significant（ P〈0.05）,and in the drug-administered group,the change level of the high-dose group of Qishenerlian Decoction was the most significant,and the difference was statistically significant（P〈0.05）.Conclusion The high dose Qishenerlian Decoction for 15 g/kg can effectively promote the expression of MMP-1,reduce the expression of TIMP-1 and reverse the hepatic fibrosis in rats.