6-BA与ZT对芫花茎段外植体组织培养的影响

Effects of 6-BA and ZT on the tissue culture of Daphne genkwa segment explant

采用芫花茎段为外植体,研究其在不同消毒方法处理下的存活情况,以及施加不同植物生长调节剂组合下芽的诱导率和生长情况。结果表明:芫花茎段外植体采用75%乙醇消毒30 s后,用0.1%氯化汞加吐温80混合液消毒12 min的处理最佳,其污染率为57.8%,死亡率为13.3%,存活率为28.9%;ZT对芫花茎段外植体上腋芽萌发的促进效果显著优于6-BA,培养基以MS+0.1 mg/L NAA+1.5 mg/L ZT处理最佳,其萌发率为92.2%,玻璃化率为22.3%,萌芽均高为2.13 cm。

The aim of this study was to evaluate the effects of different disinfection treatments on the survival of Daphne genkwa nodal segment explants and the effects of different plant growth regulator combinations on shoot induction rate andgrowth. The results showed that the best disinfection treatment was 75% ethanol disinfection for 30 s,plus 0. 1% mercuricchloride and Tween 80 mixed solution for 12 min.Under this treatment,the pollution rate reached 57. 8%, the mortality ratewas 13. 3%, and the survival rate was 28. 9%. The promoting effect of ZT on the germination of the D. genkwa segmentswas significantly better than that of 6.BA.The best medium was MS＋0. 1 mg / L NAA＋1.5 mg / L ZT as the germination ratewas 92. 2%, the vitrification rate was 22. 3%, and the average shoot height was 2. 13 cm.