黔产夜关门的显微特征与其微量元素测定

Microscopic Identification and Detection of Microelement in Guizhou Lespedeza

目的:对夜关门的显微特征及其微量元素含量进行测定,为其开发和研究提供理论依据。方法:采用显微鉴别的方法,对夜关门的根、茎、叶、种子的组织结构进行鉴别,同时利用微波消解技术-电感耦合发射光谱(ICP-OES)法对其Ca、K、Mg、Zn、Sn、Cu、Pb、As、Hg、Cd、Mn、Fe、Ag、Al、Ba、Ga、Mo、Ge、Co、Sb、Ni、Cr等元素进行含量测定。结果:鉴定出黔产夜关门的显微特征,且夜关门中含有丰富的微量元素,元素含量由高到低依次为K、Ca、Mg、Ag、Fe、Al、Mn、Zn等元素,精密度试验的RSD为0.244%~2.681%,回收率89.9%~107.0%。结论:黔产夜关门符合截叶铁扫帚的显微特征,重金属元素及微量元素的含量均符合《药用植物及制剂进出口绿色行业标准》,为黔产夜关门的综合开发利用及质量控制提供了科学依据。

Objective： To systematically study the Microscopic features and detect the microelement content of Guizhou Lespsdeza,which provide theoretical basis for future development and research of Guizhou Lespedeza. Methods： To use microscopic identification method to identify the tissue structure of the roots,stems,leaves and seeds of Guizhou Lespsdeza. and microwave digestion technology-inductively coupled emission spectroscopy（ ICP-OES） method to detect the content of its Ca,K,Mg,Zn,Sn,Cu,Pb,As,Hg,Cd,Mn,Fe,Ag,Al,Ba,Ga,Mo,Ge,Co,Sb,Ni,Cr,etc respectively according to the ＂ Green Industry Standards for Import and Export of Medicinal Plants and Preparations＂. Results： Identified the microscopic features of Guizhou Lespedeza,And it contained rich microelement. The order of element content from high to low was： K,Ca,Mg,Ag,Fe,Al,Mn,Zn and so on. The RSD of the precision test was from 0. 244% to 2. 681%,and the recovery rate was from 89. 9% to 107. 0%. Conclusions： The microscopic features of Guizhou Lespedeza are in line with Lespedeza cuneata,the content of heavy metal elements and microelement is in line with the ＂ Green Industry Standard for Import and Export of Medicinal Plants and Preparations＂. It provides a scientific basis for the comprehensive development and utilization and quality control of Guizhou Lespedeza.