秋龙筋中药制剂对链脲佐菌素诱导糖尿病大鼠的降糖作用及机制研究

Study on hypoglycemic effect and related mechanism of Qiulongjin Chinese medicine preparation on STZ-induced diabetic rats

目的探讨秋龙筋中药制剂对链脲佐菌素(STZ)诱导糖尿病大鼠的降糖作用及作用机制,为进一步深入开发提供依据。方法将24只大鼠分为正常组、模型组、阳性药组及实验组,模型组、阳性药组及实验组使用STZ诱导造模。正常组及模型组给予生理盐水,阳性药组给予盐酸二甲双胍,实验组给予秋龙筋中药制剂干预。给药结束后检测血清学指标;采集肝脏组织,检测肝糖原水平。结果实验组大鼠血中低密度脂蛋白胆固醇(LDL-C)、血清总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FBG)、丙二醛(MDA)、游离脂肪酸(FFA)、糖化血红蛋白(GHb)、一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)水平明显低于模型组,高密度脂蛋白胆固醇(HDL-C)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、肝糖原水平明显高于模型组(P<0.05)。结论采用秋龙筋中药制剂对STZ诱导糖尿病大鼠干预后,可有效降低其体内血糖水平,提高胰腺β细胞功能。

Objective To study the hypoglycemic effect and mechanism of Qiulongjin Chinese medicine preparation on STZ-induced diabetic rats.Methods Twenty-four rats were divided into the normal group,model group,positive drug group and experimental group.The model group,positive drug group and experimental group used STZ to induce the model construction.The normal group and model group were given normal saline,the positive drug group was given metformin hydrochloride,and the experimental group was given Qiulongjin Chinese medicine preparation intervention.The serological indexes were detected at the end of medication.Liver tissue was collected and liver glycogen level was detected.Results The levels of LDL-C,TC,TG,FBG,MDA,FFA,GHb,NO and TNF-αin the experimental group were significantly lower than those in the model group,while the levels of HDL-C,SOD,GSH and liver glycogen were significantly higher than those in the model group（P〈0.05）.Conclusion Adopting Qiulongjin Chinese medicine preparation for conducting the intervention on STZ-induced diabetic rats could effectively decrease the blood glucose level and increases the pancreaticβcellular function.