摘要
目的研究新型抗瘤中药制剂青蒿琥酯(ART)对T淋巴细胞表面免疫因子的影响。方法噻唑蓝(MTT)法筛选对卵巢癌3AO细胞增殖无明显影响的最高ART浓度。收集经ART预处理后的3AO细胞培养上清液(ART-S组)及连续两次再培养上清液(ART-S1组和ART-S2组),以不经ART作用的相应同步培养的3AO细胞培养上清液作对照(Con-S组、Con-S1组、Con-S2组),以完全培养基代替3AO细胞培养上清液组为正常对照组(Con组),流式细胞计数法分析各组上清液对T细胞表面CD3ε+、CD3ε+ζ+、IL-2Rα+免疫功能指标的影响。结果对3AO细胞增殖无明显影响的最高ART浓度为12.50 mg/L。Con组的CD3ε+、CD3ε+ζ+、IL-2Rα+细胞表达阳性率分别为(86.07±10.05)%、(25.09±5.84)%、(39.11±5.83)%。Con-S组3项免疫指标明显受抑制,抑制率分别为(50.17±8.02)%、(87.51±9.17)%、(64.84±9.74)%。ART直接作用后可明显下调单纯的3AO肿瘤细胞所致的3项T细胞免疫功能抑制。结论青蒿琥酯对人卵巢癌3AO细胞所致T淋巴细胞免疫功能有一定的逆转作用。
Objective To investigate the effect of new type anti-tumor Chines medicine artesunate(ART)on T lymphocyte surface immune factors by in vitro experiment.Methods The MTT method was used to screen the maximum ART concentration without significant effect on human ovary caner 3 AO cells proliferation.The 3 AO cell culture supernatant and successive two times re-culture supernatants after ART treatment were collected as the ART-S group,ART-S1 group and ART-S2 group,and corresponding synchronized 3 AO cell culture supernatant without ART action served as the control groups(Con-S group,Con-S1 group and Con-S2 group),the complete medium instituting the 3 AO cell culture supernatant served as the normal control group(Con group).The effects of supernatant in each group on T lymphocyte surface immune functional indexes of CD3ε^+,CD3ε^+ζ^+and IL-2 Rα^+were analyzed by using flow-cytometry.Results The maximum ART concentration without significant effect on 3 AO cell proliferation was 12.50 mg/L.The expression positive rates of CD3ε^+,CD3ε^+ζ^+and IL-2 Rα+cells in the Con group were(86.07±10.05)%,(25.09±5.84)% and(39.11±5.83)%respectively.Those indexes in the Con-S group were significantly repressed(P〈0.05),the suppression rates were(50.17±8.02)%,(87.51±9.17)% and(64.84±9.74)% respectively.After ART direct action,the suppression of 3 Tlymphocyte immune function caused by simply3 AO tumor cell was obviously down-regulated.Conclusion ART has a certain reversion effect on T lymphocyte immune function caused by human ovarian cancer 3 AO cells.
作者
陈晓
高英芳
张志敏
张雪玲
王岩
郝红娟
CHEN Xiao ,GAO Yingf ang ,ZHANG Zhimin ,ZHANG Xueling ,WANG Yah, HAO Hongjuan(Department of Gynecology and Obstetrics, Shijiazhuang Municipal Fourth Hospital, Shijiazhuang, Hebei 050011, China)
出处
《重庆医学》
CAS
2018年第24期3142-3145,共4页
Chongqing medicine