摘要
目的探讨小儿功能性消化不良(FD)与十二指肠嗜酸性粒细胞(EOS)增多之间的相关性。方法选取2014年1月至2017年1月在内蒙古妇幼保健院就诊的82例FD患儿为观察组,另选取同期于内蒙古妇幼保健院体检的80名健康儿童为健康对照组。观察组采取增强胃动力联合抑制胃酸治疗。经电子胃镜进行检查并取十二指肠降段黏膜组织,采取HE染色法对两组组织中的EOS进行计数统计,采用甲苯胺蓝染色计数法对组织内肥大细胞数量进行统计对比;采用酶联免疫吸附法(ELISA)对两组血浆中的白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)含量进行测量比较。结果观察组治疗前EOS、肥大细胞数均显著高于对照组(P<0.05),治疗后EOS、肥大细胞数高于对照组相应值,但差异无统计学意义(P>0.05);观察组治疗后EOS、肥大细胞数水平与治疗前相比显著降低(P<0.05)。观察组IL-6及TNF-α含量高于对照组(P<0.05),治疗后上述炎性因子水平有所下降,但仍显著高于对照组(P<0.05);观察组IL-10水平治疗前后均低于对照组(P<0.05)。EOS与IL-6、TNF-α、肥大细胞计数呈正相关关系(P<0.05),与IL-10呈负相关关系(P<0.05);肥大细胞数与IL-6、TNF-α呈正相关关系(P<0.05),与IL-10呈负相关关系(P<0.05)。结论 FD患者十二指肠黏膜EOS数量、肥大细胞数量及血清IL-6、TNF-α水平均显著提高,而IL-10水平降低,这些变化与FD的发病存在正相关关系。
Objective To investigate the correlation between infantile functional dyspepsia （FD） and increased eosinophilia （EOS） in the duodenum. Methods From January 2014 to January 2017, 82 patients with FD in Inner Mongolia Maternal and Child Health Center was selected as observation group. Another 80 healthy children in Inner Mongolia Maternal and Child Health Center was selected as healthy control group in the same period. The observation group adopted enhanced gastric motility combined with inhibition of gastric acid therapy. The duodenal descending segment mucosa was taken and examined by electronic gastroscopy, and EOS was counted in two groups by HE staining,and the number of mast cells in the tissue was counted by toluidine blue staining. The contents of IL-6, IL-10 and tumor necrosis factor-α （TNF-α） in plasma were measured by enzyme-linked immunosorbent assay （ELISA）. Results Before treatment, the number of EOS and mast cells was higher than that of the control group, the difference was statistically significant （P 〈 0.05）. After treatment, the number of EOS and mast cells was higher than that of the control group, but the difference was not statistically significant （P 〉 0.05）. The levels of EOS and mast cells in the observation group were lower than those before treatment （P 〈 0.05）, and the levels of IL-6 and TNF-α in the observation group were higher than those in the control group （P 〈 0.05）. The level of IL-10 in observation group was lower than that in control group, but still lower than that in control group （P 〈 0.05）. There was a significant positive correlation between EOS and IL-6, TNF-α and mast cell number （P 〈 0.05）, but negatively correlated with IL-10 （P 〈 0.05）. The humber of mast cells was positively correlated with IL-6 and TNF-α （P 〈 0.05）, and negatively correlated with IL-10 （P 〈 0.05）. Conclusions The number of EOS, the number of mast cells and the level of serum IL-6 and TNF-α in the duodenal mucosa of FD patients were significantly increased, and the level of IL-10 decreased. These changes were associated with the pathogenesis of FD.
作者
吴金霞
张称心
WU Jin-xia;ZHANG Chen-xin(Neonatal Department,Inner Mongolia Maternal and Child Health Center,Hohhot,Inner Mongolia,010020;Pediatric Department,The First Affiliated Hospital of Inner Mongolia Medical University,Hohhot,lnner Mongolia,010000)
出处
《现代消化及介入诊疗》
2018年第3期278-281,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
内蒙古卫生厅医疗卫生科研计划项目(2010014)
