柚皮素改善糖尿病肾病模型小鼠病情及机制研究

Study of naringenin on improving the condition of diabetic nephropathy mice and its mechanism

目的研究柚皮素对早期糖尿病肾病模型小鼠db/db 24h尿清蛋白、血糖及其分子机制的影响。方法 15只4周龄雄性小鼠分为3组:5只db/m小鼠作为正常对照组,10只4周龄高血糖且无蛋白尿的db/db小鼠分别分到空白对照组和柚皮素组。其中柚皮素组小鼠进行柚皮素50mg·kg-1·d-1混悬液灌胃,空白对照和正常对照组小鼠均进行等体积的生理盐水溶液灌胃,直至空白对照组db/db小鼠尿清蛋白显著高于正常对照组,共治疗6周。检测体质量、血糖、24尿清蛋白,硝酸还原酶法检测血清一氧化氮(NO),分光光度法检测一氧化氮合酶(NOS)。结果空白对照组较正常对照组体质量、血糖、尿清蛋白、NO和NOS显著增高,而经柚皮素治疗后的柚皮素组较空白对照组病情减轻,血糖和尿清蛋白显著减轻,氧化应激相关因子NO和NOS也明显下降。结论柚皮素可缓解抑制糖尿病肾病小鼠尿清蛋白,其机制可能通过抑制NO和NOS拮抗氧化应激,从而对糖尿病肾病治疗具有一定作用。

Objective To investigate the effect of naringenin on 24-hour urine albumin excretion,blood glucose,and its molecular mechanism in db/db mouse with early stage of diabetic nephropathy.Methods A total of 15 male 4-week-old mice were divided into three groups：the normal control group（5 cases db/m mice）,the blank control group（5 cases db/db mice）,and the naringenin group（5 cases db/db mice）.The naringenin group was intragastrically administered with naringenin 50 mg·kg^-1·d^-1 suspension.The blank control group and the normal control group were intragastrically administered with an equal volume of 0.9%sodium chloride for 6 weeks until 24-hour urine albumin excretion of the blank control group was significantly higher than that of the normal control group.The indexes of body weight,blood glucose,24-hour urine albumin excretion were tested,serum nitric oxide（NO）was measured by nitrate reductase method,and serum nitric oxide synthase（NOS）was measured by spectrophotography.Results Compared with the normal control group,the levels of body weight,blood glucose,urine albumin excretion,NO and NOS increased in the blank control group.Compared with the blank control group,the condition of diabetic nephropathy in the naringenin group was improved,and the levels of blood glucose,urine albumin excretion,NO and NOS were lower.Conclusion Naringenin can alleviate urinary albumin in diabetic nephropathy mice,and it may play an important role in the treatment of diabetic nephropathy by inhibiting NO and NOS to antagonize oxidative stress.