摘要
目的分析流感高发季节150例流感病毒核酸阴性的流感样病例(ILI)鼻咽分泌物呼吸道病毒的病毒病原谱。方法 150例ILI患者,流感病毒核酸检测均呈阴性,留取鼻咽分泌物标本,采用荧光定量PCR法检测鼻咽分泌物呼吸道合胞病毒、腺病毒、鼻病毒、冠状病毒等多种呼吸道病毒。结果 150例标本中,病毒阳性检出率29.33%(44/150)。44例中呼吸道合胞病毒22例(14.67%),其中A型3例(2.00%)、B型19例(12.67%);腺病毒9例(6.00%);鼻病毒5例(3.33%);冠状病毒5例(3.33%),其中COV-229E 1例(0.67%)、COV-NL63 1例(0.67%)、COV-OC43 3例(2.00%);混合感染3例(2.00%),分别为呼吸道合胞病毒B型合并COV-OC43、腺病毒和鼻病毒。病原谱构成分析显示,呼吸道合胞病毒A型占总体阳性标本的6.81%,B型占43.18%,腺病毒占20.45%,鼻病毒占11.36%,冠状病毒占11.36%,混合感染占6.81%。44份病毒阳性标本中,男、女性病毒阳性检出率分别为32.10%(26/81)、26.09%(18/69),二者比较,P>0.05。≤4岁、4~18岁、19~59岁、≥60岁者病毒阳性检出率分别为41.07%(23/56)、36.37%(8/22)、16.98%(9/53)、21.05%(4/19),组间比较,P均<0.05。≤4岁者多发呼吸道合胞病毒、腺病毒和鼻病毒感染,≥60岁者未检测到鼻病毒感染。结论流感高发季节血清流感病毒核酸阴性的ILI患者存在呼吸道合胞病毒、腺病毒、鼻病毒和冠状病毒等病毒感染。≤4岁ILI者多发呼吸道合胞病毒、腺病毒和鼻病毒感染。
Objective To analyze the pathogen spectrum of influenza virus-negative nasopharyngeal swabs from 150 influenza-like illness(ILI) cases during flu epidemic season. Methods Totally 150 influenza virus-negative nasopharyngeal swabs specimens were selected randomly for the respiratory syncytial virus(RSV),adenovirus(Ad V),human rhinovirus(HRV) and coronavirus(Co V) detection by using real-time PCR. Results Of 150 influenza-like cases,44 cases were virus positive with the positive rate of 29. 33%(44/150),including 22 cases of RSV infection(14. 67%),9 cases of Ad V infection(6. 00%),5 cases of HRV infection(3. 33%) and 5 cases of Co V infection(3. 33%) including 1 case of COV-229 E(0. 67%),1 case of COV-NL63(0. 67%) and 3 cases of COV-OC43(2. 00%). There were 3 cases of coinfection(2. 00%),which were RSVB co-infected with COV-OC43,Ad V and HRV,respectively. The pathogen spectrum analysis showed that RSVA accounted for 6. 81% of the total positive samples,RSVB accounted for 43. 18%,Ad V accounted for 20. 45%,HRV accounted for 11. 36%,Co V accounted for 11. 36%,and co-infection accounted for 6. 81%. In addition,the positive rate was 32. 10%(26/81) in males and 26. 09%(18/69) in females,and no significant difference was found between males and females(P〈0. 05). The positive rate was 41. 07%(23/56) in the children under four years old,36. 37%(8/22) in the age of 4-18,16. 98%(9/53) in the age of 19-59,and 21. 05%(4/19) in the old people above 60 years old,with significant difference(all P〈0. 05). RSV,Ad V and HRV were the main viruses infected in the children under 4 years old,and no HRV was detected in the old people above 60 years old. Conclusion RSV,Ad V,HRV,and Co V could be detected in the influenza-like cases,and the children under 4 years old,are more mainly infected with RSV,Ad V,and HRV.
作者
于娟
李红
饶华祥
卢囡囡
雷有菊
赵生仓
YU Juan, LI Hong, RAO Huaxiang, LU Nannan, LEI Youju, ZHAO Shengcang(Qinghai Center for Disease Control and Prevention, Xining 810007, China)
出处
《山东医药》
CAS
2018年第31期40-43,共4页
Shandong Medical Journal
基金
国家"十三五"科技重大专项资助项目(2017zx10103006)
青海省卫生计生系统重点课题(2017-wjzd-07)
关键词
流感样病例
呼吸道病毒
流感病毒
呼吸道合胞病毒
腺病毒
influenza-like illness
respiratory virus
influenza virus
respiratory syneytial virus
adenoviurs