盐生草HgNHX1基因在大麦株系中的功能验证

Functional Verification of HgNHX1 Gene Derived from Halogeton Glomeratus in Barley

为了探讨盐和干旱环境胁迫对转盐生草液泡膜型Na+/H+逆向转运蛋白基因HgNHX1大麦幼苗生长及抗逆性生理指标的影响,以转HgNHX1基因大麦及野生型株系为材料,分析盐(150mmol·L-1 NaCl)和自然干旱环境下转基因大麦幼苗的生长特性及生理指标的变化。结果表明,在盐胁迫条件下,随着胁迫时间的延长,转基因株系中HgNHX1基因相对表达量逐渐上升;干旱胁迫条件下,转基因株系中HgNHX1基因相对表达量呈先上升后下降的趋势,在处理3d时达到最高。且在胁迫处理的三个不同时间点,转基因株系的相对含水量和游离脯胺酸含量均高于野生型株系,而相对电导率和丙二醛含量均低于野生型株系。这些结果说明,转基因株系中HgNHX1基因能够应答盐和干旱胁迫,具有提高大麦耐盐、抗旱性的功能。

In order to study the effects of salt and drought stress on the growth and stress resistance physiological indices of barley seedlings transformed with a Halogeton glomeratus vacuolar Na＋/H＋antiporter gene HgNHX1,the HgNHX1 transgenic barley and wild type strains were used as materials,to analyse the changes of growth and physiological characteristics under salt（150 mmol·L-1 NaCl）stress and the natural arid environment.The results showed that under salt stress,the relative expression of HgNHX1 gene in transgenic lines increased gradually with the prolonging of stress time.With the extension of drought stress time,the relative expression of HgNHX1 gene in transgenic lines gradually increased,and reached the highest level at 3 days.At three different time points of stress treatment,the RWC and L-Pro of the transgenic lines were higher than those of the wild type,but REC and MDA were lower than those of the wild type.These results showed that HgN-HX1 gene in transgenic lines could respond to salt and drought stress,and this gene could improve the salt tolerance and drought resistance of barley.