UVA辐射对赤拟谷盗表皮超微结构影响研究（英文）

Effects of UVA radiation on cuticle microstructure of Tribolium castaneum

紫外线A(UVA)是一种重要的非生物环境因子。表皮是生物抵御UV的第一道防线,海洋生物、高等植物和人能通过改变表皮的形态和生理结构抵抗UV辐射。通过使用扫描电镜和透射电镜研究赤拟谷盗的幼虫遭受2 h或4 h的UVA辐照后表皮超微结构的变化,结果表明,随着辐照时间的增加,幼虫表皮的分泌物增加,外表皮增厚,外表皮的电子致密区增大,但内表皮细胞核,高尔基体和线粒体的形态没有受到UVA辐射的影响。这说明赤拟谷盗可通过改变表皮细胞结构抵御UVA损伤。

Ultraviolet A（ UVA） is a very important abiotic environmental factor to organisms. Cuticles are always the first line of defense to UV radiation. Changes of cuticle morphology and physiology contributing to UV resistance have been found in many marine organisms,higher plants,and human. But there were few researches about the cuticle of insects influenced by UVA. In this study,we used SEM and TEM to analyze changes of ultrastructural structure of larvae of Tribolium castaneum after 2 h and 4 h of UVA exposure. It was found that with the irradiation time increasing,boundaries of scale corresponding to the underlying epidermal cells on the dorsal surface of larvae were blurred and more secretions were deposited up to epidermal cells. In addition,the exocuticle became much thicker,and electron dense outside region of the exocuticle enlarged. However,endocuticle nuclei,Golgi apparatus and mitochondria were not influenced by UVA. These results might indicate that T. castaneum could change cuticle properties to reduce UVA damages.