利用微卫星标记对四带无须鲃不同种群的遗传质量评价

Genetic quality evaluation in different populations of tiger barb,Puntius tetrazona,using microsatellite markers

目的分析四带无须鲃封闭群及近交群的遗传质量。方法筛选多态性丰富的四带无须鲃微卫星序列,通过构建两个多重PCR反应体系再利用毛细管电泳技术进行分型,开展群体遗传多样性分析。结果野生群、WT封闭群、BT封闭群及近交群的平均等位基因数分别为6.5833、3.1667、3.0833和3.1818,平均多态信息含量分别为0.5969、0.3748、0.4159和0.4241。群体遗传质量检测分析表明:4个群体间遗传分化结果和近交群由野生群、封闭群逐渐筛选获得的过程相符。结论筛选的微卫星标记可用于四带无须鲃不同群体的遗传质量分析,为其遗传质量控制及监测提供方法基础。

Objective To analyze and evaluate the genetic quality of closed populations and inbred population of tiger barb,Puntius tetrazona. Method The microsatellite sequences with abundant polymorphism in tiger barb were screened,and two multiple PCR reaction systems were established. Then four populations were analyzed by capillary electrophoresis to identify the genotype and evaluate the genetic diversity. Result The average number of alleles in the wild group,WT closed group,BT closed group,and inbred group were 6. 5833,3. 1667,3. 0833,and 3. 1818. The average polymorphic information contents were 0. 5969,0. 3748,0. 4159 and 0. 4241,respectively. The analysis of population genetic quality showed that the result of genetic differentiation between the four groups were consistent with the process of the inbred population bred from the wild and closed group gradually. Conclusion These microsatellite markers can be used to analyze the genetic quality of different populations of tiger barb,providing a method ological basis for their genetic quality control and monitoring.