糖基氟虫腈在蓖麻体内吸收、转运与降解动态研究

Uptake,phloem transport and degradation of two glucose-fipronil conjugates in Ricinus communis

为了研究糖基氟虫腈在植物体内的吸收、转运和降解动态,为后续糖基导向农药结构设计和优化提供参考。本试验以2个糖基氟虫腈(GOTF和GOF)为研究对象,蓖麻为模式植物,研究了其韧皮部输导性、子叶吸收和培养液浓度变化,通过Kleier模型预测其吸收输导机制。结果表明:5h内韧皮部液中能检测出糖基氟虫腈,且GOF含量高于GOTF;子叶吸收表明,GOF的含量随着时间的延长而增加,在子叶中未检出GOTF;在培养液中化合物主要以糖基氟虫腈的形式存在;Kleier模型显示这两个化合物都不具有韧皮部输导性。表明GOTF在子叶中降解快,只能通过质外体途径进入韧皮部,而GOF可通过质外体途径和胞间连丝等共质体途径进入韧皮部,因此,其输导性优于GOTF。

This paper was to study the uptake,phloem transport and degradation of two glucose-fipronil conjugates in Ricinus communis and to provide an information for the design and optimization of structure of glucosyl guided pesticides.Two glucose-fipronil conjugates were selected as research object and castor bean was selected as plant material.The uptake,phloem transport and degradation of conjugates were measured,and then the Kleier model was used to predict the phloem mobility of conjugates.Results showed that the conjugates were detected in phloem sap,but the phloem mobility of GOF was more efficient than that of GOTF.Cotyledon uptake experiments showed that the amount of GOF increased within 5 h,but GOTF can＇t be detected in cotyledons.Meanwhile,glucose-fipronil conjugates were stable in incubation medium.According to predictions by the Kleier model,both GOTF and GOF were in the non-mobile molecule area.GOTF is loaded directly from the apoplast,while GOF could take the routes via direct and indirect apoplastic as well as symplastic loading.The slow hydrolysis of GOF is considered to be preferred over GOTF to be loaded in the sieve tubes.