TNFα-308G/A位点基因多态性与糖尿病性牙周炎易感性的相关性研究

Tumor necrosis factor-α-308 polymorphisim in diabetic periodontitis

目的:探讨肿瘤坏死因子-α(TNFα)基因多态性与糖尿病性牙周炎(DP)易感性的相关性。方法:电子检索MEDLINE、Web of Science、CNKI、万方以及中国生物医学文摘数据库自建库至2016-04收录的所有文献,从中收集TNFα-(308G/A)位点基因多态性与DP易感性的相关研究并进行meta分析。同时采用病例对照研究进行验证:分别收集慢性牙周炎(CP)患者(60例)、健康对照者(N)(56例)、2型糖尿病(T2DM)患者(58例)、DP患者(60例)的血液样本并采用DNA测序法检测其TNFα-(308G/A)位点的基因型。随后采用χ~2检验分析基因型和等位基因频率在各组间分布的差异,并应用非条件Logistic回归分析计算风险比(OR)及95%可信区间(95%CI),以评估各基因型与DP发病风险的相关性。结果:meta分析结果显示,TNFα-(308G/A)位点多态性可能是汉族人群DP易感性的相关因素。病例对照研究结果显示,TNFα-(308G/A)位点的3种基因型(GG、GA、AA)及等位基因(G、A)频率在各组间的分布均有统计学差异(P<0.05);携带A等位基因的人群患DP的风险明显增加(OR=5.074,95%CI:1.857~13.868,P=0.002),而且携带GA基因型的人群患DP的风险显著高于携带GG基因型的人群(OR=5.637,95%CI:1.949~16.301,P=0.001)。结论:汉族人群中TNFα-(308G/A)位点基因多态性可能是DP易感性的相关因素。

AIM： To investigate the correlation belween polymorphism of tumor necrosis factor-α （TNFα） and susceptibility to diabetic periodontitis （ DP ）. METHODS： The studies of the correlation between TNFα - （308G/A） gene polymorphism and DP susceptibility published up to April 2016 were collected by systematic searching of electronic databases MEDLINE, Web of Science, CNKI, Wan Fang Data and CBM and were processed by Meta ana- lyses. A ease -control study which consisted of 60 eases of DP, 56 healthy subjects, 60 eases of chronic periodontitis and 58 eases of type 2 diabetes mellitus were validated. The genotype of TNFα - （308 G/A） in the samples was detec- ted by DNA Sequencing. The X2 test was used to analyze the differences in frequency distribution of the genotypes and alleles among the groups. Association analysis between genotypes and the risk of DP based on unconditional logistic - regression was carried out to determine the odds ratio （OR） and 95% confidence interval （95% CI） in the different genetic models. RESULTS： Meta - analysis suggested that TNFα - （308G/A） may be associated with the risk of DP in Chinese Han population. The ease -control study showed that the frequencies of the three genotypes （G/G, G/A, A/A） and two alleles （G, A） in the TNFα - （308G/A） were significantly different among the groups （P 〈 0.05 ）. The A allele of the TNFα - （308G/A） was associated with an increased risk of DP in allele model （OR = 5. 074,95% CI：I. 857 -13. 868, P = 0.002）. In addition the model of G/A genope of TNFα - （308G/A） may be a risk factor of DP（ OR = 5. 637, 95% CI ： 1. 949 - 16. 301, P = 0.001 ）. CONCLUSION ： The polymorphisnl of TNFα - （308G/A） may be related to DP suseeptibili in Chinese Han population.