通江产辣木叶总黄酮的提取工艺优化及含量测定

Optimization of Extraction Process and Determination of Total Flavonoids from Moringa Leaf in Tongjiang District

为了考察通江产辣木叶总黄酮的提取方法和含量测定,试验采用浸渍法,以乙醇溶液作提取溶剂,分别考察乙醇浓度、料液比、温度和时间对通江产辣木叶总黄酮提取率的影响,并利用响应面法考察不同因素、不同水平对辣木叶总黄酮提取率的影响情况,筛选出辣木叶总黄酮的最佳提取工艺,测定8、9、10月通江产辣木叶总黄酮的含量。试验结果表明,辣木叶总黄酮的最佳提取条件为:料液比1∶30(g/mL)、提取时间30 min、提取温度55℃、乙醇浓度75%。在最佳提取工艺条件下,辣木叶总黄酮提取率达3.018%。利用最佳提取工艺进行8、9、10月份采摘的辣木叶总黄酮的提取,得出10月份辣木叶总黄酮的含量最高。

In this experiment,by using the Dipping method,ethanol solution as the extraction solvent,the effect of solid-to-liquid ratio,extraction time,extraction temperature,and Ethanol concentration on the extraction yield of total flavonoids were investigated using single factor experiment.Based on the results,the experimental conditions were optimized using a four-variable,three-level Box-Behnken experimental design,and the optimum conditions for the extraction were obtained as follows：the ratio of material to liquid was 1 ∶ 30（g/mL）,the extraction time was 30 min,the extraction temperature was 55 ℃,and the ethanol concentration was 75%.Under the optimum extraction conditions,the total flavonoids extraction rate was 3.018%.The extraction of flavonoids from Moringa oleifera leaves was carried out in August,September and October by the best extraction process,and the extraction rate of total flavonoids from Moringa oleifera leaves was the best in October.