AHA1蛋白对恶性黑色素瘤细胞增殖的影响

Effect of AHA1 protein on proliferation of malignant melanoma cells

目的研究AHA1蛋白对恶性黑色素瘤细胞增殖的影响。方法恶性黑色素瘤A375细胞转染真核表达载体过表达AHA1蛋白后,用CCK-8法检测细胞增殖,流式细胞术检测细胞周期变化,免疫印迹检测P21蛋白表达。结果AHA1过表达抑制A375细胞增殖(P<0.01),增加G0/G1期细胞(P<0.05)和P21表达。结论过表达AHA1通过上调P21表达、G1期阻滞,抑制恶性黑色素瘤细胞增殖。

Objective Methods To study the effect of AHA1 protein on the proliferation of malignant melanoma cells. After AHA1 protein overexpression of A375 cells was constructed by transfection with eukaryotic expression vector, the proliferation, cell cycle and P21 expression were respectively detected by CCK-8 assay, flow cytometry and Western blot.Results AHA1 overexpression inhibited proliferation, and increased G0/G1 phase cells and p21 expression. Conclusion AHA1 overexpression may inhibit proliferation of malignant melanoma cells via upregulation of p21 protein and arrest of G1 phase cells.