宫颈癌组织中人乳头瘤病毒HPV16型基因组同源序列的检测

Detection of Human Papilloma Virus 16 DMA Sequence in Biopsy Specimens of Cervical Cancer

本文报道应用Southern blot核酸分子杂交技术,以标记的国内HPV16型基因组分子为探针,共检测22例宫颈癌、4例宫颈不典型增生、6例宫颈慢性炎症,5例宫颈内膜息肉,6例正常宫颈上皮组织中的HPVl6型基因组同源序列。杂交结果表明,宫颈癌组织中HPV16型基因组同源序列阳性率为36.4％(相关序列阳性率为59.1％),非癌组织和正常宫颈上皮组织均为阴性,P<0.05具有显著性差异。同时还观察到宫颈癌病理组织学特征和组织细胞体外培养CPE与HPV16型基因组同源序列检出率呈正相关,P<0.001。更进一步证明宫颈癌发病与HPV感染密切相关。并提出HPV感染的初筛检测方法。

Using [α-42P] -dTTP-HPV 16 DNA fragments as the probe biopsy specimens (22 cases of the cervical cancer, 4 cases of the cervical dysplasia, 6 cases of the tra-chelitis, 5 cases of the polypus and 6 cases of the normal cervix) were examined for the homologous sequences of HPV 16 DNA by Southern blot nucleic acid by hybridization technique. The results indicated that 36.4% of biopsy specimens of the cervical cancer was positive for HPV 16 DNA homologous sequences (59.1% positive for HPV 16 DNA related sequences), much higher than that of non-cancerous and normal cervix (P<0.05). The pathological features and the CPE of the tissue culture of the cervical cancer closely correlated with the frequency of HPV 16 DNA homologous sequences (P<0.001). It is demonstrated that HPV infection is closely related to cervical cancer.