LRIG1在不同分化程度胃腺癌中的表达

Expression of LRIG1 in Different Differentiation of Gastric Adenocarcinoma

探讨富亮氨酸重复序列免疫球蛋白样结构域-1(LRIG1)在胃癌中的表达及其与分化程度的关系。采用免疫组化、Western blot、RT-PCR法检测LRIG1在不同分化程度胃癌组织和细胞株中的表达,并分析其表达与分化程度的关系。结果显示LRIG1在胃癌组织和细胞株中的表达低于癌旁正常胃组织和正常胃黏膜细胞株,差异有统计学意义(P<0.01);LRIG1在MGC-803、SGC-7901和HGC-27中的表达量依次降低,差异有统计学意义(P<0.01);LRIG1在胃癌的发生和发展中可能起着抑癌基因的作用。

To investigate the expression of tandem leucine-rich repeats and immunoglobulin-like domains-1（LRIG1）in gastric carcinoma,and its relationship with tumor differentiation.Immunohistochemistry,Western blot and RT-PCR methods were used to detect the expression of LRIG1 in different differentiation of gastric carcinoma tissues and cell lines,and the relationship between LRIG1 expression and tumor differentiation was analyzed.Results showed that the expression of LRIG1 in gastric carcinoma tissues and cell lines were lower than that in the adjacent normal gastric tissues and normal gastric mucosal cell lines,the difference was statistically significant（P〈0.01）;the expression of LRIG1 in MGC-803,SGC-7901 and HGC-27 cell lines was decreased,the difference was statistically significant（P〈0.01）;LRIG1 may be involved in the tumorigenesis of gastric carcinoma as a suppressor.