前列腺癌新型靶向纳米超声造影剂的合成及初步评价

Preparation of a Novel Nano Ultrasound Contrast Agent Targeting at Prostate Cancer

目的:研究制备一种前列腺癌新型靶向性纳米超声造影剂。方法:采用PEG及抗前列腺癌特异性膜抗原(PSMA)适体A10-3.2修饰多壁碳纳米管(MWCNTs)合成新型靶向纳米超声造影剂,对其进行合成验证、体外特征评价、生物相容性研究及体外显影评价。结果:Zeta电位、透射电镜扫描图(TEM)、红外分析图(FT-IR)均表明此靶向纳米造影剂合成成功。体外细胞学研究表明,该造影剂细胞毒性小、生物相容性好。体外显影结果表明,该造影剂具有良好的显影效果。结论:该新型造影剂制备成功,具备靶向显影的潜能。

Objective：To prepare a prostate cancer targeted nano-ultrasound contrast agent.Methohs：Multi-walled carbon nanotubes（MWCNTs） were modified by PEG and anti-PSMA aptamers,and the resulted products were researched on their characteristics,biological compatibility as well as development in vitro.Results：Zeta potential,transmission electron microscopy（TEM） and infrared analysis（FT-IR） results showed that this targeted nano contrast agent had been successfully synthesized.In vitro cytology showed that the contrast agent had good biocompatibility.The contrast agent had good development effects outside the body.Conclusion：The novel contrast agent has been prepared sucessfully with the potential for prostate cancer targeting development.