补中益气汤联合抗真菌药物治疗侵袭性念珠菌病的实验研究

Experimental study of Buzhongyiqi Decoction combined with anti-fungal agents treating invasive candidiasis

目的探讨补中益气汤联合抗真菌药物治疗IC小鼠的有效性和可能机制。方法 60只雄性昆明小鼠经环磷酰胺预处理后腹腔注射白念珠菌混悬液,制作IC小鼠模型。造模成功后随机分为对照组(n=30)和实验组(n=30)。两组均给予抗真菌药物灌胃治疗,实验组同时给予补中益气汤[4 g/(kg·d)]灌胃,对照组同时给予等量生理盐水灌胃。观察两组的第7天生存率;采用ELISA检测IC小鼠肝组织、血清中的TNF-α、IL-6、IL-10表达;采用流式细胞仪检测IC小鼠淋巴细胞亚群中CD3^+、CD4^+、CD8^+和CD4^+/CD8^+的表达。结果实验组第7天的生存率显著高于对照组(P<0.05)。实验组IC小鼠肝组织和血清中IL-10均显著低于对照组(P<0.05);两组IC小鼠肝组织和血清中的TNF-α、IL-6表达比较,差异均无统计学意义(P>0.05)。实验组IC小鼠淋巴细胞亚群中CD4^+/CD8^+比值显著高于对照组(P<0.05);两组IC小鼠淋巴细胞亚群中CD3^+、CD4^+、CD8^+比较,差异均无统计学意义(P>0.05)。结论补中益气汤联合抗真菌药物可以提高IC小鼠第7天的生存率,可能与提高细胞免疫功能和降低免疫抑制的发生有关。

Objective To investigate the effectiveness and possible mechanism of Buzhongyiqi Decoction combined with anti-fungal agents treating invasive candidiasis. Methods Sixty male Kunming mice were pretreated with CTX and then injected with Candida albicans suspension in abdominal cavity, the IC mice model was made. After modeled successfully, they were randomly divided into control group（n=30） and experimental group（n=30）. Two groups were given antifungal therapy, while mice in the experimental group were given Buzhongyiqi Decoction of 4 g/（kg·d）and the control group was given the same amount of normal saline. The survival rate in the seventh day in the two groups were observed. The expressions of TNF-α, IL-6 and IL-10 in liver tissues and serum of IC mice were detected by ELISA. The expressions of CD3^＋, CD4^＋, CD8^＋and CD4^＋/CD8^＋in lymphocyte subsets of IC mice were detected by flow cytometry.Results The survival rate in the seventh day of the experimental group was significantly higher than that of the control group（P〈0.05）. The expressions of IL-10 in liver tissue and serum of IC mice in the experimental group were significantly lower than those of the control group（P〈0.05）. There were no significant difference in liver tissue and serum on expressions of TNF-αand IL-6 in IC mice between the two groups（P〈0.05）. The ratio of CD4^＋/CD8^＋in lymphocyte subsets of IC mice in the experimental group were significantly higher than that of the control group（P〈0.05）. There were no significant difference of CD3^＋, CD4^＋and CD8^＋expressions in lymphocyte subsets of IC mice between the two groups（P〈0.05）. Conclusion Buzhongyiqi Decoction combined with anti-fungal agents can improve the survival rate in the seventh day of IC mice, which may be related to the enhancement of cellular immune function and the reduction of immunosuppression.