HPLC法测定藏药烈香杜鹃不同部位7个黄酮类成分的含量

Determination of seven flavones in different parts of Tebitan medicine Rhododendron anthopogonoides by HPLC

目的:建立HPLC法同时测定藏药烈香杜鹃中金丝桃苷、芦丁、槲皮苷、槲皮素、木犀草素、山柰酚和异鼠李素7个成分的含量,比较不同部位中该7个成分含量的差异。方法:采用Agilent Exlipse Extend C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.05%甲酸水溶液为流动相,梯度洗脱,流速1.0 mL·min^(-1),柱温30℃,检测波长254 nm。结果:7个黄酮类成分分离良好;在相应线性范围内线性关系良好,相关系数(r)不低于0.999 0,精密度试验的RSD均小于3.0%;平均回收率为90.1%～96.1%。不同部位中金丝桃苷、芦丁、槲皮苷、槲皮素、木犀草素、山柰酚和异鼠李素的含量范围分别为0.042～5.836、0.254～1.960、0.015～0.612、0.048～1.158、0.219～1.035、0.021～0.078、0.017～0.100 mg·g^(-1)。7个黄酮类成分的总含量,叶>嫩枝>花>老枝>根;金丝桃苷、芦丁、槲皮素和木犀草素是烈香杜鹃中含量较高的黄酮类成分,且主要集中分布于嫩枝、叶和花中。结论:该方法简便准确,可用于烈香杜鹃中黄酮类成分的含量测定和质量评价。不同部位中黄酮类成分的分布特征,为烈香杜鹃资源的合理利用提供依据。

Objective：To establish an HPLC method for the simultaneous determination of 7 flavones including hyperoside,rutin,quercitrin,quercetin,luteolin,kaempferol and isorhamnetin in Tebitan medicine Rhododendron anthopogonoides. And to compare the differences of the content of the above seven flavones in different parts of the plant. Methods：The HPLC method was performed on an Agilent Exlipse Extend C18 column（250 mm×4.6 mm,5 μm） with a gradient elution of methanol and 0.05% formic acid solution. The flow rate was 1.0 mL·min-1. The column temperature was 30 ℃ and the detection wavelength was set at 254 nm. Results：Excellent chromatographic separation was achieved with good linearity（r ≥0.999 0） within the linear range of the seven flavones. RSD of the precision test was less than 3.0%. The average recoveries of the seven flavones were in the range of 90.1%-96.1%. The contents of hyperoside,rutin,quercitrin,quercetin,luteolin,kaempferol and isorhamnetin in different parts of plant were 0.042-5.836,0.254-1.960,0.015-0.612,0.048-1.158,0.219-1.035,0.021-0.078 and 0.017-0.100 mg·g-1,respectively. The results showed that the total content of seven flavones decreased as follows：leaves twigs flowersold branchesroots. Hypericum,rutin,quercetin and luteolin were the major flavones in Rhododendron anthopogonoides,which mainly distributed in twigs,leaves and flowers. Conclusion：The established HPLC method is simple and accurate. It can be applied for the flavones determination and quality evaluation of Rhododendron anthopogonoides. The distribution feature of flavones in the plant can provide the basis for rational use of resources.