超高效液相色谱法同时测定广藿香中6个成分的含量

Simultaneous determination of 6 components in Pogostemonis Herba by ultra performance liquid chromatography

目的:建立同时测定广藿香中新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇和广藿香酮的超高效液相色谱(UPLC)分析方法。方法:采用Waters BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速0.4 mL·min^(-1);柱温30℃;检测波长309nm。结果:新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇、广藿香酮的线性范围分别为2.18～109.00、8.66～433.11、2.12～106.00、1.81～90.40、0.90～45.00、1.09～54.24μg·mL^(-1)(r^2>0.999 7);平均回收率(n=9)为99.1%～100.7%,精密度、重复性、稳定性均符合有关规定;10批样品中6个成分的含量范围分别为0.137～0.959、0.980～5.877、0.306～1.363、0.273～1.169、0.136～0.387、0.292～1.619 mg·g^(-1)。结论:本法经方法学验证,可用于广藿香质量控制。

Absract Objective：To establish a UPLC method for simultaneous determination of vicenin-2,verbascoside,isoverbascoside,crenatoside,pachypodol and pogostone in Pogostemonis Herba. Methods：Analysis was performed on a Waters BEH C18 column（100 mm×2.1 mm,1.7μm）. The mobile phase consisted of acetonitrile -0.1% phosphoric acid in gradient mode. The flow rate was 0.4 mL·min-1. The column temperature was maintained at 30 ℃ and the detection wavelength was set at 309 nm. Results：Vicenin-2,verbascoside,isoverbascoside,crenatoside,pachypodol and pogostone showed a good linear relationship within the ranges of 2.18-109.00 μg·mL-1,8.66-433.11μg·mL-1,2.12-106.00 μg·mL-1,1.81-90.40 μg·mL-1,0.90-45.00 μg·mL-1 and 1.09-54.24 μg·mL-1,respectively（r20.999 7）. The average recoveries of the six compounds were within 99.1%-100.7%. Results of precision,stability and reproducibility test were satisfactory. The contents of six components in ten batches of samples were 0.137-0.959 mg·g-1,0.980-5.877 mg·g-1,0.306-1.363 mg·g-1,0.273-1.169 mg·g-1,0.136-0.387 mg·g-1 and 0.292-1.619 mg·g-1,respectively. Conclusion：The established method can be used for the quality control of Pogostemonis Herba.