摘要
目的:建立简便快速的离子迁移谱法(IMS法)用于现场快速检测抗风湿类中成药中添加的非甾体抗炎药(布洛芬、萘普生、双氯芬酸钠、吲哚美辛、对乙酰氨基酚),并对抗风湿中成药样品进行检测。方法:采用正离子模式对样品进行分析,取适量样品加甲醇溶解,0.45μm滤膜过滤,直接进样。离子迁移谱工作参数:源电压3 kV,迁移管电压8 kV,迁移管温度180℃。结果:本文所建立的方法可以在2~3 min内完成样品筛查,且不需要任何样品前处理步骤;对阴性样品和模拟阳性样品进行分析可见,样品基质(含48种常见抗风湿药材)不干扰测定,方法专属性良好。方法检测下限范围为0.06~0.33μg·mL^(-1),耐用性良好。结论:本方法快速、灵敏,结果准确,为抗风湿类中成药非法添加的现场筛查提供了一种简便易行的手段。
Objective:To establish a simple and rapid ion mobility spectrometry(IMS)method used for in-situ rapid detection of non-steroidal anti-inflammatory drugs(NSAIDs)such as ibuprofen,naproxen,diclofenac sodium,indomethacin and acetaminophen illegally added in anti-rheumatism traditional patent medicines.Methods:The sample was firstly dissolved in methanol,filtered by 0.45 μm microfiltration membranes,then directly injected and analyzed in the positive mode.The experimental parameter was as follows:source voltage was 3 kV,drift tube voltage was 8 kV,and temperature of drift tube was 180 ℃.Results:By utilization of this method,screening of illegal additions in anti-rheumatism Chinese traditional patent medicines can be finished within 2-3 minutes with no pretreatment required.Besides,both negative sample and simulative positive sample were detected by the proposed method,and there was no interference observed in sample matrix including 48 kinds of anti-rheumatism herbs;the method was specific.Under the optimized conditions of good robustness,limit of detection in the range 0.06-0.33 μg·mL-1 was obtained.Conclusion:The method is rapid and sensitive,thus provides a simple and convenient method for in-situ screening of illegally addition detection in anti-rheumatism Chinese traditional patent medicines.
作者
孙轶东
李梦娇
马红云
商惠敏
高敬林
马海艳
蒋晔
SUN Yi-dong;LI Meng-jiao;MA Hong-yun;SHANG Hui-min;GAO Jing-lin;MA Hai-yan;JIANG Ye(Pharmacy College Hebei Medical University,Shijiazhuang 050017,China;Fourth Hospital of Hebei,Medical University,Shijiazhuang 050017,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2018年第8期1410-1418,共9页
Chinese Journal of Pharmaceutical Analysis
基金
河北省省级大学生创业创新训练项目(基金编号:USIP2016019)
关键词
离子迁移谱
非法添加
抗风湿中成药
非甾体抗炎药
现场筛查
快速检测
ion mobility spectrometry ( IMS )
illegally addition
anti-rheumatism Chinese traditional patent medicines
non-steroidal anti-inflammatory drugs ( NSAIDs )
rapid in-situ screening
rapid detection