便携式离子迁移谱现场快速筛查抗风湿类中成药中添加的非甾体抗炎药

Rapid in-situ screening of non-steroidal anti-inflammatory drugs added in anti-rheumatism Chinese traditional patent medicines by a portable ion mobility spectrum

目的:建立简便快速的离子迁移谱法(IMS法)用于现场快速检测抗风湿类中成药中添加的非甾体抗炎药(布洛芬、萘普生、双氯芬酸钠、吲哚美辛、对乙酰氨基酚),并对抗风湿中成药样品进行检测。方法:采用正离子模式对样品进行分析,取适量样品加甲醇溶解,0.45μm滤膜过滤,直接进样。离子迁移谱工作参数:源电压3 kV,迁移管电压8 kV,迁移管温度180℃。结果:本文所建立的方法可以在2～3 min内完成样品筛查,且不需要任何样品前处理步骤;对阴性样品和模拟阳性样品进行分析可见,样品基质(含48种常见抗风湿药材)不干扰测定,方法专属性良好。方法检测下限范围为0.06～0.33μg·mL^(-1),耐用性良好。结论:本方法快速、灵敏,结果准确,为抗风湿类中成药非法添加的现场筛查提供了一种简便易行的手段。

Objective：To establish a simple and rapid ion mobility spectrometry（IMS）method used for in-situ rapid detection of non-steroidal anti-inflammatory drugs（NSAIDs）such as ibuprofen,naproxen,diclofenac sodium,indomethacin and acetaminophen illegally added in anti-rheumatism traditional patent medicines.Methods：The sample was firstly dissolved in methanol,filtered by 0.45 μm microfiltration membranes,then directly injected and analyzed in the positive mode.The experimental parameter was as follows：source voltage was 3 kV,drift tube voltage was 8 kV,and temperature of drift tube was 180 ℃.Results：By utilization of this method,screening of illegal additions in anti-rheumatism Chinese traditional patent medicines can be finished within 2-3 minutes with no pretreatment required.Besides,both negative sample and simulative positive sample were detected by the proposed method,and there was no interference observed in sample matrix including 48 kinds of anti-rheumatism herbs;the method was specific.Under the optimized conditions of good robustness,limit of detection in the range 0.06-0.33 μg·mL-1 was obtained.Conclusion：The method is rapid and sensitive,thus provides a simple and convenient method for in-situ screening of illegally addition detection in anti-rheumatism Chinese traditional patent medicines.