灵芝提取物中多糖质量控制方法初探

Preliminary study on quality standard for polysaccharide in Ganoderma lucidum extracts

目的:探讨灵芝提取物中多糖质量控制方法的建立。方法:3批灵芝提取物通过水提醇沉后,得到灵芝粗多糖。采用三氟乙酸进行全水解,以1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化后,采用HPLC法于250 nm波长处检测单糖组成及含量。采用Shodex HQ 806+804+803凝胶渗透色谱柱串联,示差折光检测器-多角度激光散射(RI-MALLS)联用技术测定灵芝多糖重均相对分子质量(Mw)。结果:3批灵芝提取物多糖主要由葡萄糖、半乳糖、甘露糖及葡萄糖醛酸组成,其中葡萄糖含量最高。MALLS测定结果显示,灵芝多糖主要呈现出2个峰,3批次样品峰1的Mw分别为1.2×10~6、4.6×10~6、4.1×10~6,峰2的Mw分别为3.2×10~4、4.6×10~4、4.0×10~4。结论:本文对灵芝提取物中多糖的单糖组成及Mw进行初步研究,为建立灵芝提取物中多糖的质量控制方法提供技术参考。

Objective：To establish the quality standard for polysaccharide in Ganoderma lucidum extracts.Methods：The polysaccharides from 3 Ganoderma lucidum extracts were extracted with hot water,followed by precipitating with ethanol.The samples were first hydrolyzed with trifluoroacetic acid,and then derivatized with 1-phenyl-3-methyl-5-pyrazolone（PMP）.The content of monosaccharide was determined by HPLC at 250 nm.The Shodex HQ 806＋804＋803 gel permeation chromatographic column was used,and the weight average relative molecular mass（Mw）of Ganoderma lucidum polysaccharide was determined by differential refraction detector and multi angle laser scattering（RI-MALLS）.Results：The polysaccharide of 3 Ganoderma lucidum extracts were composed of mannose（Man）,glucose（Glc）,galactose（Gal） and Glucuronic acid（Glc A）and the content of Glc was the highest.The Mw of polysaccharide determined by MALLS showed two main peaks.The Mw of peak 1 in 3 samples was 1.2×106,4.6×106 and 4.1×106 and the Mw of peak 2 was 3.2×104,4.6×104 and 4.0×104,respectively.Conclusion：The monosaccharide composition and Mw of polysaccharide in Ganoderma lucidum extracts were studied,and this research can offer reference for quality control of polysaccharide in Ganoderma lucidum extracts.