-Gx暴露家兔肺组织核因子κB表达变化

Changes of nuclear factor κB expression in rabbit lung induced by -Gx exposure

目的观察-Gx暴露家兔肺脏组织K基因结合核因子（nuclearfactore-genebinding，NF-κB）的表达水平，探讨-Gx暴露的力学因素诱导机体肺组织产生NF-κB的病理生理学意义。方法按随机数字表法，将20只家兔随机分为对照组和-Gx暴露组（实验组）共两组，每组10只。-Gx平台暴露后运用免疫荧光、Westernblot检测肺组织NF-κB的表达情况。数据处理采用SPSS22．0统计软件，定量数据以均数±标准差表示，对照组与暴露组肺组织切片的阳性细胞数差异比较采用成组t检验。结果NF-kB表达阳性的细胞在荧光显微镜下观察，可呈现出明亮的绿色荧光，与对照组相比，实验组动物肺组织切片NF-kB表达阳性细胞数显著增多（t=2．69，P〈0．01）。Westernblot检测结果显示，与对照组相比，实验组肺组织中NF-κB的表达水平显著增强（P〈0．01）。结论-Gx暴露增加了肺组织NF-κB的表达水平，-Gx暴露的环境下机体炎性因子表达上调和氧化应激水平增高，提示肺脏受到一定的损伤。

Objective To explore the physiological and pathological mechanisms of nuclear factor-κ-gene binding （NF-κB） changes induced by the mechanics effect of --Gx exposure by observing the NF-κB expression level in rabbit lung. Methods Twenty rabbits were randomly divided into control group and -Gx exposure group （experimental group） by random number table method. Each group had 10 rabbits. After exposing to the -Gx, the expression of NF-κB in rabbits＇ lung tissue was detected by western blot and immunofluoreseence. All data were processed by SPSS 22.0 statistical software package, and the measurement data were expressed by （x- s）. The difference on the positive cells in lung tissue sliees was compared between control and experimental groups by t-test. Results By the fluorescence microscope observation, the positive cells expressed by NF-κB showed bright green fluorescence. Compared with the control group, the number of positive cells expressed by NF-κB in the lung biopsy of the experimental group was significantly increased （t = 2.69, P 〈0.01）. Western blot test results showed that the expression levels of NF-κB in lung tissue of experimentalgroup were significantly increased as compared with that of control group （P〈0.01）. Conclusions -Gx exposure results in the increment of NF-κB expression in rabbitsp lung tissue. The up regulation of inflammatory factors and increased oxidative stress under -Gx exposure may indicate certain injury to lung.